Nucleotide and deduced amino acid sequences of the envelope 1 gene of 51 isolates of hepatitis C virus and the use of reagents derived from these sequences in diagnostic methods and vaccines

ABSTRACT

The nucleotide and deduced amino acid sequences of 51 cDNAs are disclosed where each cDNA encodes the envelope 1 gene of an isolate of hepatitis C virus (HCV). The invention relates to the oligonucleotides, peptides and recombinant envelope 1 proteins derived from these sequences and their use in diagnostic methods and vaccines.

FIELD OF INVENTION

The present invention is in the field of hepatitis virology. The invention relates to the complete nucleotide and deduced amino acid sequences of the envelope 1 (E1) gene of 51 hepatitis C virus (HCV) isolates from around the world and the grouping of these isolates into twelve distinct HCV genotypes. More specifically, this invention relates to oligonucleotides, peptides and recombinant proteins derived from the envelope 1 gene sequences of the 51 isolates of hepatitis C virus and to diagnostic methods and vaccines which employ these reagents.

BACKGROUND OF INVENTION

Hepatitis C, originally called non-A, non-B hepatitis, was first described in 1975 as a disease serologically distinct from hepatitis A and hepatitis B (Feinstone, S. M. et al. (1975) N. Engl. J. Med. 292:767-770). Although hepatitis C was (and is) the leading type of transfusion-associated hepatitis as well as an important part of community-acquired hepatitis, little progress was made in understanding the disease until the recent identification of hepatitis C virus (HCV) as the causative agent of hepatitis C via the cloning and sequencing of the HCV genome (Choo, A. L. et al. (1989) Science 288:359-362). The sequence information generated by this study resulted in the characterization of HCV as a small, enveloped, positive-stranded RNA virus and led to the demonstration that HCV is a major cause of both acute and chronic hepatitis worldwide (Weiner, A. J. et al. (1990) Lancet 335:1-3). These observations, combined with studies showing that over 50% of acute cases of hepatitis C progress to chronicity with 20% of these resulting in cirrhosis and an undetermined proportion progressing to liver cancer, have led to tremendous efforts by investigators within the hepatitis C field to develop diagnostic assays and vaccines which can detect and prevent hepatitis C infection.

The cloning and sequencing of the HCV genome by Choo et al. (1989) has permitted the development of serologic tests which can detect HCV or antibody to HCV (Kuo, G. et al. (1989) Science 244:362-364). In addition, the work of Choo et al. has also allowed the development of methods for detecting HCV infection via amplification of HCV RNA sequences by reverse transcription and cDNA polymerase chain reaction (RT-PCR) using primers derived from the HCV genomic sequence (Weiner, A. J. et al.). However, although the development of these diagnostic methods has resulted in improved diagnosis of HCV infection, only approximately 60% of cases of hepatitis C are associated with a factor identified as contributing to transmission of HCV (Alter, M. J. et al. (1989) JAMA 262:1201-1205). This observation suggests that effective control of hepatitis C transmission is likely to occur only via universal pediatric vaccination as has been initiated recently for hepatitis B virus. Unfortunately, attempts to date to protect chimpanzees from hepatitis C infection via administration of recombinant vaccines have had only limited success. Moreover, the apparent genetic heterogeneity of HCV, as indicated by the recent assignment of all available HCV isolates to one of four genotypes, I-IV (Okamoto, H. et al. (1992) J. Gen. Virol; 73:673-679), presents additional hurdles which must be overcome in order to develop accurate and effective diagnostic assays and vaccines.

For example, one possible obstacle to the development of effective hepatitis C vaccines would arise if the observed genetic heterogeneity of HCV reflects serologic heterogeneity. In such a case, the most genetically diverse strains of HCV may then represent different serotypes of HCV with the result being that infection with one strain may not protect against infection with another. Indeed, the inability of one strain to protect against infection with another strain was recently noted by both Farci et al. (Farci, P. et al. (1992) Science 258:135-140) and Prince et al. (Prince, A. M. et al. (1992) J. Infect. Dis. 165:438-443), each of whom presented evidence that while infection with one strain of HCV does modify the degree of the hepatitis C associated with the reinfection, it does not protect against reinfection with a closely related strain. The genetic heterogeneity among different HCV strains also increases the difficulty encountered in developing RT-PCR assays to detect HCV infection since such heterogeneity often results in false-negative results because of primer and template mismatch. In addition, currently used serologic tests for detection of HCV or for detection of antibody to HCV are not sufficiently well developed to detect all of the HCV genotypes which might exist in a given blood sample. Finally, in terms of choosing the proper treatment modality to combat hepatitis infection, the inability of presently available serologic assays to distinguish among the various genotypes of HCV represents a significant shortcoming in that recent reports suggest that an HCV-infected patient's response to therapy might be related to the genotype of the infectious virus (Yoshioka, K. et al. (1992) Hepatology 16:293-299; Kanai, K. et al. (1992) Lancet 339:1543; Lan, J. Y. N. et al. (1992) Hepatology 16:209A). Indeed, the data presented in the above studies suggest that the closely related genotypes I and II are less responsive to interferon therapy than are the closely related genotypes III and IV. Moreover, preliminary data by Pozzato et al. (Pozzato, G. et al. (1991) Lancet 338:509) suggests that different genotypes may be associated with different types or degrees of clinical disease. Taken together, these studies suggest that before effective vaccines against HCV infection can be developed, and indeed, before more accurate and effective methods for diagnosis and treatment of HCV infection can be produced, one must obtain a greater knowledge about the genetic and serologic diversity of HCV isolates.

In a recent attempt to gain an understanding of the extent of genetic heterogeneity among HCV strains, Bukh et al. carried out a detailed analysis of HCV isolates via the use of PCR technology to amplify different regions of the HCV genome (Bukh, J. et al. (1992a) Proc. Natl. Acad. Sci. 89:187-191). Following PCR amplification, the 5'-noncoding (5' NC) portion of the genomes of various HCV isolates were sequenced and it was found that primer pairs designed from conserved regions of the 5' NC region of the HCV genome were more sensitive for detecting the presence of HCV than were primer pairs representing other portions of the genome (Bukh, J. et al. (1992b) Proc. Natl. Acad. Sci. U.S.A. 89:4942-4946). In addition, the authors noted that although many of the HCV isolates examined could be classified into the four genotypes described by Okamoto et al. (1992), other previously undescribed genotypes emerged based on genetic heterogeneity observed in the 5' NC region of the various isolates. One of the most prominent of these newly noted genotypes comprised a group of related viruses that contained the most genetically divergent 5' NC regions of those studied. This group of viruses, tentatively classified as a fifth genotype, are very similar to strains recently described by others (Cha, T. -A et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:7144-7148; Chan, S-W. et al. (1992) J. Gen. Virol., 73:1131-1141 and Lee, C-H et al. (1992) J. Clin. Microbio. 30:1602-1604). In addition, at least four more putative genotypes were identified thereby providing evidence that the genetic heterogeneity of HCV was more extensive than previously appreciated.

However, while the studies of Bukh et al. (1992a and b) provided new and useful information on the genetic heterogeneity of HCV, it is widely appreciated by those skilled in the art that the three structural genes of HCV, core (C), envelope (El) and envelope 2/nonstructural 1 (E2/NS1) are the most important for the development of serologic diagnostics and vaccines since it is the product of these genes that constitutes the hepatitis C virion. Thus, a determination of the nucleotide sequence of one or all of the structural genes of a variety of HCV isolates would be useful in designing reagents for use in diagnostic assays and vaccines since a demonstration of genetic heterogeneity in a structural gene(s) of HCV isolates might suggest that some of the HCV genotypes represent distinct serotypes of HCV based upon the previously observed relationship between genetic heterogeneity and serologic heterogeneity among another group of single-stranded, positive-sense RNA viruses, the picornaviruses (Ruechert, R. R. "Picornaviridae and their replication", in Fields, B. N. et al., eds. Virology, New York: Raven Press, Ltd. (1990) 507-548).

SUMMARY OF INVENTION

The present invention relates to 51 cDNAs, each encoding the complete nucleotide sequence of the envelope 1 (E1) gene of an isolate of human hepatitis C virus (HCV).

The present invention also relates to the nucleic acid and deduced amino acid sequences of these E1 cDNAs.

It is an object of this invention to provide synthetic nucleic acid sequences capable of directing production of recombinant E1 proteins, as well as equivalent natural nucleic acid sequences. Such natural nucleic acid sequences may be isolated from a cDNA or genomic library from which the gene capable of directing synthesis of the E1 proteins may be identified and isolated. For purposes of this application, nucleic acid sequence refers to RNA, DNA, cDNA or any synthetic variant thereof which encodes for peptides.

The invention also relates to the method of preparing recombinant E1 proteins derived from the E1 cDNA sequences by cloning the nucleic acid and inserting the cDNA into an expression vector and expressing the recombinant protein in a host cell.

The invention also relates to isolated and substantially purified recombinant E1 proteins and analogs thereof encoded by the E1 cDNAs.

The invention further relates to the use of recombinant E1 proteins as diagnostic agents and as vaccines.

The invention also relates to the use of single-stranded antisense poly- or oligonucleotides derived from the E1 cDNAs to inhibit the expression of the hepatitis C E1 gene.

The invention further relates to multiple computer-generated alignments of the nucleotide and deduced amino acid sequences of the 51 E1 cDNAs. These multiple sequence alignments serve to highlight regions of homology and non-homology between different sequences and hence, can be used by one skilled in the art to design peptides and oligonucleotides useful as reagents in diagnostic assays and vaccines.

The invention therefore also relates to purified and isolated peptides and analogs thereof derived from E1 CDNA sequences.

The invention further relates to the use of these peptides as diagnostic agents and vaccines.

The present invention also encompasses methods of detecting antibodies specific for hepatitis C virus in biological samples. The methods of detecting HCV or antibodies to HCV disclosed in the present invention are useful for diagnosis of infection and disease caused by HCV and for monitoring the progression of such disease. Such methods are also useful for monitoring the efficacy of therapeutic agents during the course of treatment of HCV infection and disease in a mammal.

The invention also provides a kit for the detection of antibodies specific for HCV in a biological sample where said kit contains at least one purified and isolated peptide derived from the E1 cDNA sequences.

The invention further provides isolated and purified genotype-specific oligonucleotides and analogs thereof derived from E1 cDNA sequences.

The invention also relates to a method for detecting the presence of hepatitis C virus in a mammal, said method comprising analyzing the RNA of a mammal for the presence of hepatitis C virus. The invention further relates to a method for determining the genotype of hepatitis C virus present in a mammal. This method is useful in determining the proper course of treatment for an HCV-infected patient.

The invention also provides a diagnostic kit for the detection of hepatitis C virus in a biological sample. The kit comprises purified and isolated nucleic acid sequences useful as primers for reverse-transcription polymerase chain reaction (RT-PCR) analysis of RNA for the presence of hepatitis C virus.

The invention further provides a diagnostic kit for the determination of the genotype of a hepatitis C virus present in a mammal. The kit comprises purified and isolated nucleic acid sequences useful as primers for RT-PCR analysis of RNA for the presence of HCV in a biological sample and purified and isolated nucleic acid sequences useful as hybridization probes in determining the genotype of the HCV isolate detected in PCR.

This invention also relates to pharmaceutical compositions for use in prevention or treatment of hepatitis C in a mammal.

DESCRIPTION OF FIGURES

FIGS. 1A-1 through 1H-5 show computer generated sequence alignments of the nucleotide sequences of the 51 HCV E1 cDNAs. The single letter abbreviations used for the nucleotides shown in FIGS. 1A-1 through 1H-5 are those standardly used in the art. FIGS. 1A-1 through 1A-10 show the alignment of SEQ ID NOs:1-8 to produce a consensus sequence for genotype I/1a. FIGS. 1B-1 through 1B-10 show the alignment of SEQ ID NOs:9-25 to produce a consensus sequence for genotype II/1b. FIGS. 1C-1 through 1C-5 show the alignment of SEQ ID NOs:26-29 to produce a consensus sequence for genotype III/2a. FIGS. 1D- through 1D-5 show the alignment of SEQ ID NOs:30-33 to produce a consensus sequence for genotype IV/2b. FIGS. 1E-1 through 1E-5 show the alignment of SEQ ID NOs:35-39 to produce a consensus sequence for genotype V/3a. FIGS. 1F-1 through 1F-3 show the computer alignment of SEQ ID NOs:42-43 to produce a consensus sequence for genotype 4C. FIGS. 1G-1 through 1G-5 show the alignment of SEQ ID NOs:45-50 to produce a consensus sequence for genotype 5a. The nucleotides shown in capital letters in the consensus sequences of FIGS. 1A-1 through 1G-5 are those conserved within a genotype while nucleotides shown in lower case letters in the consensus sequences are those variable within a genotype. In addition, in FIGS. 1A-1 through 1E-5 and 1G-1 through 1G-5, when the lower case letter is shown in a consensus sequence, the lower case letter represents the nucleotide found most frequently in the sequences aligned to produce the consensus sequence. In FIG. 1E-1 through 1E-5 the lower case letters shown in the consensus sequence are nucleotides in SEQ ID NO:42 which differ from nucleotides found in the same positions in SEQ ID NO:43. Finally, a hyphen at a nucleotide position in the consensus sequences in FIGS. 1A-1 through 1G-5 indicates that two nucleotides were found in equal numbers at that position in the aligned sequences. In the aligned sequences, nucleotides are shown in lower case letters if they differed from the nucleotides of both adjacent isolates. FIGS. 1H-1 through 1H-5 show the alignment of the consensus sequences of FIGS. 1A-1 through 1G-5 with SEQ ID NO:34 (genotype 2c), SEQ ID NO:40 (genotype 4a), SEQ ID NO:41 (genotype 4b), SEQ ID NO:44 (genotype 4d) and SEQ ID NO:51 (genotype 6a) to produce a consensus sequence for all twelve genotypes. This consensus sequence is shown as the bottom line of FIGS. 1H-1 through 1H-5 where the nucleotides shown in capital letters are conserved among all genotypes and a blank space indicates that the nucleotide at that position is not conserved among all genotypes.

FIGS. 2A-1 through 2H-2 show computer alignments of the deduced amino acid sequences of the 51 HCV E1 cDNAs. The single letter abbreviations used for the amino acids shown in FIGS. 2A-H follow the conventional amino acid shorthand for the twenty naturally occurring amino acids. FIGS. 2A-1 through 2A-4 show the alignment of SEQ ID NOs:52-59 to produce a consensus sequence for genotype I/1a. FIGS. 2B-1 through 2C-2 show the alignment of SEQ ID NOs:60-76 to produce a consensus sequence for genotype II/1b. FIGS. 2C-1 and 2C-2 show the alignment of SEQ ID NOs:77-80 to produce a consensus sequence for genotype III/2a. FIGS. 2D-1 and 2D-2 show the alignment of SEQ ID NOs:81-84 to produce a consensus sequence for genotype IV/2b. FIGS. 2E-1 and 2E-2 show the alignment of SEQ ID NOs:86-90 to produce a consensus sequence for genotype V/3a. FIG. 2F shows the computer alignment of SEQ ID NOS:93-94 to produce a consensus sequence for genotype 4c. FIG. 2G-1 and 2E-2 show the alignment of SEQ ID NOs:96-101 to produce a consensus sequence for genotype 5a. The amino acids shown in capital letters in the consensus sequences of FIGS. 2A-1 through 2G-2 are those conserved within a genotype while amino acids shown in lower case letters in the consensus sequences are those variable within a genotype. In addition, in FIGS. 2A-1 through 2E-2 and 2G-1 through 2G-2 when the lower case letter is shown in a consensus sequence, the letter represents the amino acid found most frequently in the sequences aligned to produce the consensus sequence. In FIG. 2F-1, the lower case letters shown in the consensus sequence are amino acids in SEQ ID NO:93 which differ from amino acids found in the same positions in SEQ ID NO:94. Finally, a hyphen at an amino acid position in the consensus sequences of FIGS. 2A-1 through 2G-2 indicates that two amino acids were found in equal numbers at that position in the aligned sequences. In the aligned sequences, amino acids are shown in lower case letters if they differed from the amino acids of both adjacent isolates. FIGS. 2H-1 and 2H-2 show the alignment of the consensus sequences of FIGS. 2A-1 through 2G-2 with SEQ ID NO:85 (genotype 2c), SEQ ID NO:91 (genotype 4a), SEQ ID NO:92 (genotype 4b), SEQ ID NO:95 (genotype 4d) and SEQ ID NO:102 (genotype 6a) to produce a consensus sequence for all twelve genotypes. This consensus sequence is shown as the bottom line of FIGS. 2H-1 and 2H-2 where the amino acids shown in capital letters are conserved among all genotypes and a blank space indicates that the amino acid at that position is not conserved among all genotypes.

FIGS. 3A and 3B show multiple sequence alignment of the deduced amino acid sequence of the E1 gene of 51 HCV isolates collected worldwide. The consensus sequence of the E1 protein is shown in boldface (top). In the consensus sequence cysteine residues are highlighted with stars, potential N-linked glycosylation sites are underlined, and invariant amino acids are capitalized, whereas variable amino acids are shown in lower case letters. In the alignment, amino acids are shown in lower case letters if they differed from the amino acid of both adjacent isolates. Amino acid residues shown in bold print in the alignment represent residues which at that position in the amino acid sequence are genotype-specific. Amino acids that were invariant among all HCV isolates are shown as hyphens (-) in the alignment. Amino acid positions correspond to those of the HCV prototype sequence (HCV-1, Choo, L. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2451-2455) with the first amino acid of the E1 protein at position 192. The grouping of isolates into 12 genotypes (I/1a, II/1b, III/2a, IV/2b, V/3a, 2c, 4a, 4b, 4c, 4d, 5a and 6a) is indicated.

FIG. 4 shows a dendrogram of the genetic relatedness of the twelve genotypes of HCV based on the percent amino acid identity of the E1 gene of the HCV genome. The twelve genotypes shown are designated as I/1a, II/1b, III/2a, IV/2b, V/3a, 2c, 4a, 4b, 4c, 4d, 5a and 6a. The shaded bars represent a range showing the maximum and minimum homology between the amino acid sequence of any one isolate of the genotype indicated and the amino acid sequence of any other isolate.

FIG. 5 shows the distribution of the complete E1 gene sequence of 74 HCV isolates into the twelve HCV genotypes in the 12 countries studied. For 51 of these HCV isolates, including 8 isolates of genotype I/1a, 17 isolates of genotype II/1b and 26 isolates comprising the additional 10 genotypes, the complete E1 gene sequence was determined. In the remaining 23 isolates, all of genotypes I/1a and II/1b, the genotype assignment was based on only a partial E1 gene sequence. The partially sequenced isolates did not represent additional genotypes in any of the 12 countries. The number of isolates of a particular genotype is given in each of the 12 countries studied. For ease of viewing, those genotypes designated by two terms (e.g., I/1a) are indicated by the latter term (e.g. 1a). The designations used for each country are: Denmark (DK); Dominican Republic (DR); Germany (D); Hong Kong (HK); India (IND); Sardinia, Italy (S); Peru (P); South Africa (SA); Sweden (SW); Taiwan (T); United States (US); and Zaire (Z) . National borders depicted in this figure represent those existing at the time of sampling.

DETAILED DESCRIPTION OF INVENTION

The present invention relates to 51 cDNAs, each encoding the complete nucleotide sequence of the envelope 1 (E1) gene of an isolate of human hepatitis C virus (HCV). The cDNAs of the present invention were obtained as follows. Viral RNA was extracted from serum collected from humans infected with hepatitis C virus and the viral RNA was then reverse transcribed and amplified by polymerase chain reaction using primers deduced from the sequence of the HCV strain H-77 (Ogata, N. et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88:3392-3396). The amplified cDNA was then isolated by gel electrophoresis and sequenced.

The present invention further relates to the nucleotide sequences of the cDNAs encoding the E1 gene of the 51 HCV isolates. These nucleotide sequences are shown in the sequence listing as SEQ ID NO:1 through SEQ ID NO:51.

The abbreviations used for the nucleotides are those standardly used in the art.

The deduced amino acid sequence of each of SEQ ID NO:1 through SEQ ID NO:51 are presented in the sequence listing as SEQ ID NO:52 through SEQ ID NO:102 where the amino acid sequence in SEQ ID NO:52 is deduced from the nucleotide sequence shown in SEQ ID NO:1, the amino acid sequence shown in SEQ ID NO:53 is deduced from the nucleotide sequence shown in SEQ ID NO:2 and so on. The deduced amino acid sequence of each of SEQ ID Nos:52-102 starts at nucleotide 1 of the corresponding sequence shown in SEQ ID NOs:1-51 and extends 595 nucleotides.

The three letter abbreviations used in SEQ ID Nos:52-102 follow the conventional amino acid shorthand for the twenty naturally occurring amino acids.

Preferably, the E1 proteins or peptides of the present invention are substantially homologous to, and most preferably biologically equivalent to, the native HCV E1 proteins or peptides. By "biologically equivalent" as used throughout the specification and claims, it is meant that the compositions are immunogenicically equivalent to the native E1 proteins or peptides. The E1 proteins or peptides of the present invention may also stimulate the production of protective antibodies upon injection into a mammal that would serve to protect the mammal upon challenge with HCV. By "substantially homologous" as used throughout the ensuing specification and claims to describe E1 proteins and peptides, it is meant a degree of homology in the amino acid sequence to the native E1 proteins or peptides. Preferably the degree of homology is in excess of 90, preferably in excess of 95, with a particularly preferred group of proteins being in excess of 99 homologous with the native E1 proteins or peptides.

Variations are contemplated in the cDNA sequences shown in SEQ ID NO:1 through SEQ ID NO:51 which will result in a DNA sequence that is capable of directing production of analogs of the corresponding envelope 1 (E1) protein shown in SEQ ID NO:52 through SEQ ID NO:102. It should be noted that the DNA sequences set forth above represent a preferred embodiment of the present invention. Due to the degeneracy of the genetic code, it is to be understood that numerous choices of nucleotides may be made that will lead to a DNA sequence capable of directing production of the instant E1 protein or its analogs. As such, DNA sequences which are functionally equivalent to the sequence set forth above or which are functionally equivalent to sequences that would direct production of analogs of the E1 proteins produced pursuant to the amino acid sequences set forth above, are intended to be encompassed within the present invention.

The term analog as used throughout the specification or claims to describe the E1 proteins or peptides of the present invention, includes any protein or peptide having an amino acid residue sequence substantially identical to a sequence specifically shown herein in which one or more residues have been conservatively substituted with a biologically equivalent residue. Examples of conservative substitutions include the substitution of one-polar (hydrophobic) residue such as isoleucine, valine, leucine or methionine for another, the substitution of one polar (hydrophilic) residue for another such as between arginine and lysine, between glutamine and asparagine, between glycine and serine, the substitution of one basic residue such as lysine, arginine or histidine for another, or the substitution of one acidic residue, such as aspartic acid or glutamic acid for another.

The phrase "conservative substitution" also includes the use of a chemically derivatized residue in place of a non-derivatized residue provided that the resulting protein or peptide is biologically equivalent to the native E1 protein or peptide.

"Chemical derivative" refers to an E1 protein or peptide having one or more residues chemically derivatized by reaction of a functional side group. Examples of such derivatized molecules, include but are not limited to, those molecules in which free amino groups have been derivatized to form amine hydrochlorides, p-toluene sulfonyl groups, carbobenzoxy groups, t-butyloxycarbonyl groups, chloracetyl groups or formyl groups. Free carboxyl groups may be derivatized to form salts, methyl and ethyl esters or other types of esters or hydrazides. Free hydroxyl groups may be derivatized to form O-acyl or O-alkyl derivatives. The imidazole nitrogen of histidine may be derivatized to form N-imbenzylhistidine. Also included as chemical derivatives are those proteins or peptides which contain one or more naturally-occurring amino acid derivatives of the twenty standard amino acids. For examples: 4-hydroxyproline may be substituted for proline; 5-hydroxylysine may be substituted for lysine; 3-methylhistidine may be substituted for histidine; homoserine may be substituted for serine; and ornithine may be substituted for lysine. The E1 protein or peptide of the present invention also includes any protein or peptide having one or more additions and/or deletions or residues relative to the sequence of a peptide whose sequence is shown herein, so long as the peptide is biologically equivalent to the native E1 protein or peptide.

The present invention also includes a recombinant DNA method for the manufacture of HCV E1 proteins. In this method, natural or synthetic nucleic acid sequences may be used to direct the production of E1 proteins.

In one embodiment of the invention, the method comprises:

(a) preparation of a nucleic acid sequence capable of directing a host organism to produce HCV E1 protein;

(b) cloning the nucleic acid sequence into a vector capable of being transferred into and replicated in a host organism, such vector containing operational elements for the nucleic acid sequence;

(c) transferring the vector containing the nucleic acid and operational elements into a host organism capable of expressing the protein;

(d) culturing the host organism under conditions appropriate for amplification of the vector and expression of the protein; and

(e) harvesting the protein.

In another embodiment of the invention, the method for the recombinant DNA synthesis of an HCV E1 protein encoded by any one of the nucleic acid sequences shown in SEQ ID NOs:1-51 comprises:

(a) culturing a transformed or transfected host organism containing a nucleic acid sequence capable of directing the host organism to produce a protein, under conditions such that the protein is produced, said protein exhibiting substantial homology to a native E1 protein isolated from HCV having the amino acid sequence according to any one of the amino acid sequences shown in SEQ ID NOs:52-102 or combinations thereof.

In one embodiment, the RNA sequence of an HCV isolate was isolated and cloned to cDNA as follows. Viral RNA is extracted from a biological sample collected from human subjects infected with hepatitis C and the viral RNA is then reverse transcribed and amplified by polymerase chain reaction using primers deduced from the sequence of HCV strain H-77 (Ogata et al. (1991)). Preferred primer sequences are shown as SEQ ID NOs:103-108 in the sequence listing. Once amplified, the PCR fragments are isolated by gel electrophoresis and sequenced.

The vectors contemplated for use in the present invention include any vectors into which a nucleic acid sequence as described above can be inserted, along with any preferred or required operational elements, and which vector can then be subsequently transferred into a host organism and replicated in such organisms. Preferred vectors are those whose restriction sites have been well documented and which contain the operational elements preferred or required for transcription of the nucleic acid sequence.

The "operational elements" as discussed herein include at least one promoter, at least one operator, at least one leader sequence, at least one terminator codon, and any other DNA sequences necessary or preferred for appropriate transcription and subsequent translation of the vector nucleic acid. In particular, it is contemplated that such vectors will contain at least one origin of replication recognized by the host organism along with at least one selectable markers and at least one promoter sequence capable of initiating transcription of the nucleic acid sequence.

In construction of the recombinant for expression cloning vector of the present invention, it should additionally be noted that multiple copies of the nucleic acid sequence and its attendant operational elements may be inserted into each vector. In such an embodiment, the host organism would produce greater amounts per vector of the desired E1 protein. The number of multiple copies of the DNA sequence which may be inserted into the vector is limited only by the ability of the resultant vector due to its size, to be transferred into and replicated and transcribed in an appropriate host microorganism.

In another embodiment, restriction digest fragments containing a coding sequence for E1 proteins can be inserted into a suitable expression vector that functions in prokaryotic or eukaryotic cells. By suitable is meant that the vector is capable of carrying and expressing a complete nucleic acid sequence coding for E1 protein. Preferred expression vectors are those that function in a eukaryotic cell. Examples of such vectors include but are not limited to vaccinia virus vectors, adenovirus or herpes viruses. A preferred vector is the baculovirus transfer vector, pBlueBac.

In yet another embodiment, the selected recombinant expression vector may then be transfected into a suitable eukaryotic cell system for purposes of expressing the recombinant protein. Such eukaryotic cell systems include but are not limited to cell lines such as HeLa, MRC-5 or Cv-1. A preferred eukaryotic cell system is SF9 insect cells.

The expressed recombinant protein may be detected by methods known in the art including, but not limited to, Coomassie blue staining and Western blotting.

The present invention also relates to substantially purified and isolated recombinant E1 proteins. In one embodiment, the recombinant protein expressed by the SF9 cells can be obtained as a crude lysate or it can be purified by standard protein purification procedures known in the art which may include differential precipitation, molecular sieve chromatography, ion-exchange chromatography, isoelectric focusing, gel electrophoresis and affinity and immunoaffinity chromatography. The recombinant protein may be purified by passage through a column containing a resin which has bound thereto antibodies specific for the open reading frame (ORF) protein.

The present invention further relates to the use of recombinant E1 proteins as diagnostic agents and vaccines. In one embodiment, the expressed recombinant proteins of this invention can be used in immunoassays for diagnosing or prognosing hepatitis C in a mammal. For the purposes of the present invention, "mammal" as used throughout the specification and claims, includes, but is not limited to humans, chimpanzees, other primates and the like. In a preferred embodiment, the immunoassay is useful in diagnosing hepatitis C infection in humans.

Immunoassays of the present invention may be a radioimmunoassay, Western blot assay, immunofluorescent assay, enzyme immunoassay, chemiluminescent assay, immunohistochemical assay and the like. Standard techniques known in the art for ELISA are described in Methods in Immunodiagnosis, 2nd Edition, Rose and Bigazzi, eds., John Wiley and Sons, 1980 and Campbell et al., Methods of Immunology, W. A. Benjamin, Inc., 1964, both of which are incorporated herein by reference. Such assays may be a direct, indirect, competitive, or noncompetitive immunoassay as described in the art (Oellerich, M. 1984. J. Clin. Chem. Clin. BioChem 22:895-904) Biological samples appropriate for such detection assays include, but are not limited to serum, liver, saliva, lymphocytes or other mononuclear cells.

In a preferred embodiment, test serum is reacted with a solid phase reagent having surface-bound recombinant HCV E1 protein as an antigen. The solid surface reagent can be prepared by known techniques for attaching protein to solid support material. These attachment methods include non-specific adsorption of the protein to the support or covalent attachment of the protein to a reactive group on the support. After reaction of the antigen with anti-HCV antibody, unbound serum components are removed by washing and the antigen-antibody complex is reacted with a secondary antibody such as labelled anti-human antibody. The label may be an enzyme which is detected by incubating the solid support in the presence of a suitable fluorimetric or calorimetric reagent. Other detectable labels may also be used, such as radiolabels or colloidal gold, and the like.

The HCV E1 protein and analogs thereof may be prepared in the form of a kit, alone, or in combinations with other reagents such as secondary antibodies, for use in immunoassays.

In yet another embodiment the recombinant E1 proteins or analogs thereof can be used as a vaccine to protect mammals against challenge with Hepatitis C. The vaccine, which acts as an immunogen, may be a cell, cell lysate from cells transfected with a recombinant expression vector or a culture supernatant containing the expressed protein. Alternatively, the immunogen is a partially or substantially purified recombinant protein.

While it is possible for the immunogen to be administered in a pure or substantially pure form, it is preferable to present it as a pharmaceutical composition, formulation or preparation.

The formulations of the present invention, both for veterinary and for human use, comprise an immunogen as described above, together with one or more pharmaceutically acceptable carriers and optionally other therapeutic ingredients. The carrier(s) must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. The formulations may conveniently be presented in unit dosage form and may be prepared by any method well-known in the pharmaceutical art.

All methods include the step of bringing into association the active ingredient with the carrier which constitutes one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product into the desired formulation.

Formulations suitable for intravenous intramuscular, subcutaneous, or intraperitoneal administration conveniently comprise sterile aqueous solutions of the active ingredient with solutions which are preferably isotonic with the blood of the recipient. Such formulations may be conveniently prepared by dissolving the solid active ingredient in water containing physiologically compatible substances such as sodium chloride (e.g. 0.1-2.0 m), glycine, and the like, and having a buffered pH compatible with physiological conditions to produce an aqueous solution, and rendering said solution sterile. These may be present in unit or multi-dose containers, for example, sealed ampoules or vials.

The formulations of the present invention may incorporate a stabilizer. Illustrative stabilizers are preferably incorporated in an amount of 0.11-10,000 parts by weight per part by weight of immunogens. If two or more stabilizers are to be used, their total amount is preferably within the range specified above. These stabilizers are used in aqueous solutions at the appropriate concentration and pH. The specific osmotic pressure of such aqueous solutions is generally in the range of 0.1-3.0 osmoles, preferably in the range of 0.8-1.2. The pH of the aqueous solution is adjusted to be within the range of 5.0-9.0, preferably within the range of 6-8. In formulating the immunogen of the present invention, anti-adsorption agent may be used.

Additional pharmaceutical methods may be employed to control the duration of action. Controlled release preparations may be achieved through the use of polymer to complex or adsorb the proteins or their derivatives. The controlled delivery may be exercised by selecting appropriate macromolecules (for example polyester, polyamino acids, polyvinyl pyrrolidone, ethylenevinylacetate, methylcellulose, carboxymethylcellulose, or protamine sulfate) and the concentration of macromolecules as well as the methods of incorporation in order to control release. Another possible method to control the duration of action by controlled-release preparations is to incorporate the proteins, protein analogs or their functional derivatives, into particles of a polymeric material such as polyesters, polyamino acids, hydrogels, poly(lactic acid) or ethylene vinylacetate copolymers. Alternatively, instead of incorporating these agents into polymeric particles, it is possible to entrap these materials in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly (methylmethacylate) microcapsules, respectively, or in colloidal drug delivery systems, for example, liposomes, albumin microspheres, microemulsions, nanoparticles, and nanocapsules or in macroemulsions.

When oral preparations are desired, the compositions may be combined with typical carriers, such as lactose, sucrose, starch, talc, magnesium stearate, crystalline cellulose, methyl cellulose, carboxymethyl cellulose, glycerin, sodium alginate or gum arabic among others.

The proteins of the present invention may be supplied in the form of a kit, alone, or in the form of a pharmaceutical composition as described above.

Vaccination can be conducted by conventional methods. For example, the immunogen or immunogens (i.e. the E1 protein may be administered alone or in combination with the E1 proteins derived from other isolates of HCV) can be used in a suitable diluent such as saline or water, or complete or incomplete adjuvants. Further, the immunogen(s) may or may not be bound to a carrier to make the protein(s) immunogenic. Examples of such carrier molecules include but are not limited to bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), tetanus toxoid, and the like. The immunogen(s) can be administered by any route appropriate for antibody production such as intravenous, intraperitoneal, intramuscular, subcutaneous, and the like. The immunogen(s) may be administered once or at periodic intervals until a significant titer of anti-HCV antibody is produced. The antibody may be detected in the serum using an immunoassay.

The administration of the immunogen(s) of the present invention may be for either a prophylactic or therapeutic purpose. When provided prophylactically, the immunogen(s) is provided in advance of any exposure to HCV or in advance of any symptom of any symptoms due to HCV infection. The prophylactic administration of the immunogen serves to prevent or attenuate any subsequent infection of HCV in a mammal. When provided therapeutically, the immunogen(s) is provided at (or shortly after) the onset of the infection or at the onset of any symptom of infection or disease caused by HCV. The therapeutic administration of the immunogen(s) serves to attenuate the infection or disease.

In addition to use as a vaccine, the compositions can be used to prepare antibodies to HCV E1 proteins. The antibodies can be used directly as antiviral agents. To prepare antibodies, a host animal is immunized using the E1 proteins native to the virus particle bound to a carrier as described above for vaccines. The host serum or plasma is collected following an appropriate time interval to provide a composition comprising antibodies reactive with the E1 protein of the virus particle. The gamma globulin fraction or the IgG antibodies can be obtained, for example, by use of saturated ammonium sulfate or DEAE Sephadex, or other techniques known to those skilled in the art. The antibodies are substantially free of many of the adverse side effects which may be associated with other anti-viral agents such as drugs.

The antibody compositions can be made even more compatible with the host system by minimizing potential adverse immune system responses. This is accomplished by removing all or a portion of the Fc portion of a foreign species antibody or using an antibody of the same species as the host animal, for example, the use of antibodies from human/human hybridomas. Humanized antibodies (i.e., nonimmunogenic in a human) may be produced, for example, by replacing an immunogenic portion of an antibody with a corresponding, but nonimmunogenic portion (i.e., chimeric antibodies). Such chimeric antibodies may contain the reactive or antigen binding portion of an antibody from one species and the Fc portion of an antibody (nonimmunogenic) from a different species. Examples of chimeric antibodies, include but are not limited to, non-human mammal-human chimeras, rodent-human chimeras, murine-human and rat-human chimeras (Robinson et al., International Patent Application 184,187; Taniguchi M., European Patent Application 171,496; Morrison et al., European Patent Application 173,494; Neuberger et al., PCT Application WO 86/01533; Cabilly et al., 1987 Proc. Natl. Acad. Sci. USA 84:3439; Nishimura et al., 1987 Canc. Res. 47:999; Wood et al., 1985 Nature 314:446; Shaw et al., 1988 J. Natl. Cancer Inst. 80:15553, all incorporated herein by reference).

General reviews of "humanized" chimeric antibodies are provided by Morrison S., 1985 Science 229:1202 and by Oi et al., 1986 BioTechniques 4:214.

Suitable "humanized" antibodies can be alternatively produced by CDR or CEA substitution (Jones et al., 1986 Nature 321:552; Verhoeyan et al., 1988 Science 239:1534; Biedleret al. 1988 J. Immunol. 141:4053, all incorporated herein by reference).

The antibodies or antigen binding fragments may also be produced by genetic engineering. The technology for expression of both heavy and light cain genes in E. coli is the subject of the PCT patent applications; publication number WO 901443, WO 901443, and WO 9014424 and in Huse et al., 1989 Science 246:1275-1281.

The antibodies can also be used as a means of enhancing the immune response. The antibodies can be administered in amount similar to those used for other therapeutic administrations of antibody. For example, normal immune globulin is administered at 0.02-0.1 ml/1b body weight during the early incubation period of other viral diseases such as rabies, measles, and hepatitis B to interfere with viral entry into cells. Thus, antibodies reactive with the HCV E1 protein can be passively administered alone or in conjunction with another antiviral agent to a host infected with an HCV to enhance the immune response and/or the effectiveness of an antiviral drug.

Alternatively, anti-HCV E1 antibodies can be induced by administered anti-idiotype antibodies as immunogens. Conveniently, a purified anti-HCV E1 antibody preparation prepared as described above is used to induce anti-idiotype antibody in a host animal, the composition is administered to the host animal in a suitable diluent. Following administration, usually repeated administration, the host produces anti-idiotype antibody. To eliminate an immunogenic response to the Fc region, antibodies produced by the same species as the host animal can be used or the Fc region of the administered antibodies can be removed. Following induction of anti-idiotype antibody in the host animal, serum or plasma is removed to provide an antibody composition. The composition can be purified as described above for anti-HCV E1 antibodies, or by affinity chromatography using anti-HCV E1 antibodies bound to the affinity matrix. The anti-idiotype antibodies produced are similar in conformation to the authentic HCV E1 protein and may be used to prepare an HCV vaccine rather than using an HCV E1 protein.

When used as a means of inducing anti-HCV virus antibodies in an animal, the manner of injecting the antibody is the same as for vaccination purposes, namely intramuscularly, intraperitoneally, subcutaneously or the like in an effective concentration in a physiologically suitable diluent with or without adjuvant. One or more booster injections may be desirable.

The HCV E1 proteins of the invention are also intended for use in producing antiserum designed for pre- or post-exposure prophylaxis. Here an E1 protein, or mixture of E1 proteins is formulated with a suitable adjuvant and administered by injection to human volunteers, according to known methods for producing human antisera. Antibody response to the injected proteins is monitored, during a several-week period following immunization, by periodic serum sampling to detect the presence of anti-HCV E1 serum antibodies, using an immunoassay as described herein.

The antiserum from immunized individuals may be administered as a pre-exposure prophylactic measure for individuals who are at risk of contracting infection. The antiserum is also useful in treating an individual post-exposure, analogous to the use of high titer antiserum against hepatitis B virus for post-exposure prophylaxis.

For both in vivo use of antibodies to HCV virus-like particles and proteins and anti-idiotype antibodies and diagnostic use, it may be preferable to use monoclonal antibodies. Monoclonal anti-HCV E1 protein antibodies or anti-idiotype antibodies can be produced as follows. The spleen or lymphocytes from an immunized animal are removed and immortalized or used to prepare hybridomas by methods known to those skilled in the art. (Goding, J. W. 1983. Monoclonal Antibodies: Principles and Practice, Pladermic Press, Inc., New York, N.Y., pp. 56-97). To produce a human-human hybridoma, a human lymphocyte donor is selected. A donor known to be infected with HCV (where infection has been shown for example by the presence of anti-virus antibodies in the blood or by virus culture) may serve as a suitable lymphocyte donor. Lymphocytes can be isolated from a peripheral blood sample or spleen cells may be used if the donor is subject to splenectomy. Epstein-Barr virus (EBV) can be used to immortalize human lymphocytes or a human fusion partner can be used to produce human-human hybridomas. Primary in vitro immunization with peptides can also be used in the generation of human monoclonal antibodies.

Antibodies secreted by the immortalized cells are screened to determine the clones that secrete antibodies of the desired specificity. For monoclonal anti-E1 antibodies, the antibodies must bind to HCV E1 protein. For monoclonal anti-idiotype antibodies, the antibodies must bind to anti-E1 protein antibodies. Cells producing antibodies of the desired specify are selected.

The present invention also relates to the use of single-stranded antisense poly- or oligonucleotides derived from nucleotide sequences substantially homologous to those shown in SEQ ID NOs:1-51 to inhibit the expression of hepatitis C E1 genes. By substantially homologous as used throughout the specification and claims to describe the nucleic acid sequences of the present invention, is meant a level of homology between the nucleic acid sequence and the SEQ ID NOs. referred to in that sentence. Preferably, the level of homology is in excess of 80%, more preferably in excess of 90%, with a preferred nucleic acid sequence being in excess of 95% homologous with the DNA sequence shown in the indicated SEQ ID NO. These anti-sense poly- or oligonucleotides can be either DNA or RNA. The targeted sequence is typically messenger RNA and more preferably, a single sequence required for processing or translation of the RNA. The anti-sense poly- or oligonucleotides can be conjugated to a polycation such as polylysine as disclosed in Lemaitre, M. et al. ((1989) Proc. Natl. Acad. Sci. USA 84:648-652) and this conjugate can be administrated to a mammal in an amount sufficient to hybridize to and inhibit the function of the messenger RNA.

The present invention further relates to multiple computer-generated alignments of the nucleotide and deduced amino acid sequences shown in SEQ ID NOs:1-102. Computer analysis of the nucleotide sequences shown in SEQ ID NOs.:1-51 and of the deduced amino acid sequences shown in SEQ ID NOs:52-102 can be carried out using commercially available computer programs known to one skilled in the art.

In one embodiment, computer analysis of SEQ ID NOs.:1-51 by the program GENALIGN (Intelligenetics, Inc. Mountainview, Calif.) results in distribution of the 51 sequences into twelve genotypes based upon the degree of variation of the sequences. For the purposes of the present invention, the nucleotide sequence identity of E1 cDNAs of HCV isolates of the same genotype is in the range of about 85% to about 100% whereas the identity of E1 cDNA sequences of different genotypes is in the range of about 50% to about 80%.

The grouping of SEQ ID NOs.:1-51 into twelve HCV genotypes is shown below.

    ______________________________________     SEQ ID NOs:     Genotypes     ______________________________________     1-8             I/1a      9-25           II/1b     26-29           III/2a     30-33           IV/2b     34              2c     35-39           V/3a     40              4a     41              4b     42-43           4c     44              4d     45-50           5a     51              6a     ______________________________________

For those genotypes containing more than one E1 nucleotide sequence, computer alignment of the constituent nucleotide sequences of the genotype was conducted using GENALIGN in order to produce a consensus sequence for each genotype. These alignments and their resultant consensus sequences are shown in FIGS. 1A-1 through 1G-5 for the seven genotypes (I/1a, II/1b, III/2a, IV/2b, V/3a, 4c and 5a) which comprise more than one nucleotide sequence. Further alignment of the consensus sequences of FIGS. 1A-1 through 1G-5 with SEQ ID NO:34 (genotype 2c), SEQ ID NO:40 (genotype 4a), SEQ ID NO:41 (genotype 4b), SEQ ID NO:44 (genotype 4d) and SEQ ID NO:51 (genotype 6a) produces a consensus sequence for all twelve genotypes as shown in FIGS. 1H-1 through 1H-5. The multiple alignments of nucleotide sequences shown in FIGS. 1A-1 through 1H-5 serve to highlight regions of homology and non-homology between different sequences and hence, can be used by one skilled in the art to design oligonucleotides useful as reagents in diagnostic assays for HCV.

Examples of purified and isolated oligonucleotide sequences provided by the present invention are shown as SEQ ID NOs:109-135. The oligonucleotides shown in SEQ ID NOs:109-135 are useful as "genotype-specific" primers and probes since these oligonucleotides can hybridize specifically to the nucleotide sequence of the E1 gene of HCV isolates belonging to a single genotype. The genotype-specificity of the oligonucleotides shown in SEQ ID NOs:109-135 is as follows: SEQ ID NOs:109-110 are specific for genotype I/1a; SEQ ID NOs:111-112 are specific for genotype II/1b; SEQ ID NOs:113-114 are specific for genotype III/2a; SEQ ID NOs:115-116 are specific for genotype IV/2b; SEQ ID NOs:117-119 are specific for genotype 2c; SEQ ID NOs:120-122 are specific for genotype V/3a; SEQ ID NOs:123-124 are specific for genotype 4a; SEQ ID NOs:125-125 are specific for genotype 4b; SEQ ID NOs:127-128 are specific for genotype 4c; SEQ ID NOs:129-130 are specific for genotype 4d; SEQ ID NOs:131-132 are specific for genotype 5a and SEQ ID NOs:133-135 are specific for genotype 6a.

The oligonucleotides of this invention can be synthesized using any of the known methods of oligonucleotide synthesis (e.g., the phosphodiester method of Agarwal et al. 1972, Agnew. Chem. Int. Ed. Engl. 11:451, the phosphotriester method of Hsiung et al. 1979, Nucleic Acids Res 6:1371, or the automated diethylphosphoramidite method of Baeucage et al. 1981, Tetrahedron Letters 22:1859-1862), or they can be isolated fragments of naturally occurring or cloned DNA. In addition, those skilled in the art would be aware that oligonucleotides can be synthesized by automated instruments sold by a variety of manufacturers or can be commercially custom ordered and prepared. In a preferred embodiment, SEQ ID NO:103 through SEQ ID NO:135 are synthetic oligonucleotides.

The present invention also relates to a method for detecting the presence of HCV in a mammal, said method comprising analyzing the RNA of a mammal for the presence of hepatitis C virus.

The RNA to be analyzed can be isolated from serum, liver, saliva, lymphocytes or other mononuclear cells as viral RNA, whole cell RNA or as poly(A)⁺ RNA. Whole cell RNA can be isolated by methods known to those skilled in the art. Such methods include extraction of RNA by differential precipitation (Birnbiom, H. C. (1988) Nucleic Acids Res., 16:1487-1497), extraction of RNA by organic solvents (Chomczynski, P. et al. (1987) Anal. Biochem., 162:156-159) and extraction of RNA with strong denaturants (Chirgwin, J. M. et al. (1979) Biochemistry, 18:5294-5299). Poly(A)⁺ RNA can be selected from whole cell RNA by affinity chromatography on oligo-d(T) columns (Aviv, H. et al. (1972) Proc. Natl. Acad. Sci., 69:1408-1412). A preferred method of isolating RNA is extraction of viral RNA by the quanidium-phenol-chloroform method of Bukh et al. (1992a).

The methods for analyzing the RNA for the presence of HCV include Northern blotting (Alwine, J. C. et al. (1977) Proc. Natl. Acad. Sci., 74:5350-5354), dot and slot hybridization (Kafatos, F. C. et al. (1979) Nucleic Acids Res., 7:1541-1522), filter hybridization (Hollander, M. C. et al. (1990) Biotechniques; 9:174-179), RNase protection (Sambrook, J. et al. (1989) in "Molecular Cloning, A Laboratory Manual", Cold Spring Harbor Press, Plainview, N.Y.) and reverse-transcription polymerase chain reaction (RT-PCR) (Watson, J. D. et al. (1992) in "Recombinant DNA" Second Edition, W. H. Freeman and Company, New York). A preferred method is RT-PCR. In this method, the RNA can be reverse transcribed to first strand cDNA using a primer or primers derived from the nucleotide sequences shown in SEQ ID NOs:1-51. A preferred primer for reverse transcription is that shown in SEQ ID NO:104. Once the cDNAs are synthesized, PCR amplification is carried out using pairs of primers designed to hybridize with sequences in the HCV E1 cDNA which are an appropriate distance apart (at least about 50 nucleotides) to permit amplification of the cDNA and subsequent detection of the amplification product. Each primer of a pair is a single-stranded oligonucleotide of about 20 to about 60 bases in length where one primer (the "upstream" primer) is complementary to the original RNA and the second primer (the "downstream" primer) is complementary to the first strand of cDNA generated by reverse transcriptions of the RNA. The target sequence is generally about 100 to about 300 base pairs long but can be as large as 500-1500 base pairs. Optimization of the amplification reaction to obtain sufficiently specific hybridization to the E1 nucleotide sequence is well within the skill in the art and is preferably achieved by adjusting the annealing temperature.

In one embodiment, the primer pairs selected to amplify E1 cDNAs are universal primers. By "universal", as used to describe primers throughout the claims and specification, is meant those primer pairs which can amplify E1 gene fragments derived from an HCV isolate belonging to any one of the twelve genotypes of HCV described herein. Purified and isolated universal primers are used in Example 1 of the present invention and are shown as SEQ ID NOs:103-108 where SEQ ID NOs:103 and 104 represent one pair of primers, SEQ ID NOs:105 and 106 represent a second pair of primers and SEQ ID NOs:107-108 represent a third pair of primers.

In an alternative embodiment, primer pairs selected to amplify E1 cDNAs are genotype-specific primers. In the present invention, genotype-specific primer pairs can readily be derived from the following genotype-specific nucleotide domains: nucleotides 197-238 and 450-480 of the consensus sequence of genotype I/1a shown in FIGS. 1A-4 and 1A-8; nucleotides 197-238 and 450-480 of the consensus sequence of genotype II/1b shown in FIGS. 1B-4 and 1B-8; nucleotides 199-238 and 438-480 of the consensus sequence of genotype III/2a shown in FIGS. 1C-2 and 1C-4; nucleotides 124-177 and 450-480 of the consensus sequence of genotype IV/2b shown in FIGS. 1D-2 and ID-4; nucleotides 124-177, 193-238 and 436-480 of SEQ ID NO:34 (genotype 2C); nucleotides 168-207, 294-339 and 406-480 of the consensus sequence of genotype V/3a shown in FIGS. 1E-2, 1E-3 and IE-4; nucleotides 145-183 and 439-480 of SEQ ID NO:40 (genotype 4a); nucleotides 168-207 and 432-480 of SEQ ID NO:41 (genotype 4b); nucleotides 130-183 and 450-480 of the consensus sequence of genotype 4c shown in FIGS. 1F-1 and 1F-2; nucleotides 130-183 and 450-480 of SEQ ID NO:44 (genotype 4d); nucleotides 166-208 and 437-480 of the consensus sequence of genotype 5a shown in FIGS. 1G-2 and IG-4 and nucleotides 168-207, 216-252 and 429-480 of SEQ ID NO:51 (genotype 6a). One skilled in the art would readily appreciate that in a pair of genotype-specific primers, each primer is derived from different genotype-specific nucleotide domains indicated above for a given genotype. Also, as described earlier, it is understood by one skilled in the art that each pair of primers comprises one primer which is complementary to the original viral RNA and the other which is complementary to the first strand of cDNA generated by reverse transcription of the viral RNA. For example, in a pair of genotype-specific primers for genotype 4b, one primer would have a nucleotide sequence derived from region 168-207 of SEQ ID NO:40 and the other primer would have a nucleotide sequence which is the complement of region 432-480 of SEQ ID NO:40. One skilled in the art would readily recognize that such genotype specific domains would also be useful in designing oligonucleotides for use as genotype-specific hybridization probes. Indeed, the sequences of such genotype-specific hybridization probes are disclosed later in the specification.

The amplification products of PCR can be detected either directly or indirectly. In one embodiment, direct detection of the amplification products is carried out via labelling of primer pairs. Labels suitable for labelling the primers of the present invention are known to one skilled in the art and include radioactive labels, biotin, avidin, enzymes and fluorescent molecules. The derived labels can be incorporated into the primers prior to performing the amplification reaction. A preferred labelling procedure utilizes radiolabeled ATP and T4 polynucleotide kinase (Sambrook, J. et al. (1989) in "Molecular Cloning, A Laboratory Manual", Cold Spring Harbor Press, Plainview, N.Y.). Alternatively, the desired label can be incorporated into the primer extension products during the amplification reaction in the form of one or more labelled dNTPs. In the present invention, the labelled amplified PCR products can be detected by agarose gel electrophoresis followed by ethidum bromide staining and visualization under ultraviolet light or via direct sequencing of the PCR-products.

In yet another embodiment, unlabelled amplification products can be detected via hybridization with labelled nucleic acid probes radioactively labelled or, labelled with biotin, in methods known to one skilled in the art such as dot and slot blot hybridization (Kafatos, F. C. et al. (1979) or filter hybridization (Hollander, M. C. et al. (1990)).

In one embodiment, the nucleic acid sequences used as probes are selected from, and substantially homologous to, SEQ ID NOs:1-51. Such probes are useful as universal probes in that they can detect in PCR-amplification products of E1 cDNAs of an HCV isolate belonging to any of the twelve HCV genotypes disclosed herein. The size of these probes can range from about 200 to about 500 nucleotides.

In an alternative embodiment, the present invention relates to a method for determining the genotype of a hepatitis C virus present in a mammal where said method comprises:

(a) amplifying RNA of a mammal via RT-PCR to produce amplification products;

(b) contacting said products with at least one genotype-specific oligonucleotide; and

(c) detecting complexes of said products which bind to said oligonucleotide(s).

In this method, one embodiment of said amplification step is carried out using the universal primers (SEQ ID NO:103 through SEQ ID NO:108) as disclosed above. In step (b) of this method, the nucleic acid sequences used as probes are substantially homologous to the sequences shown in SEQ ID NOs:109-135. The probes disclosed in SEQ ID NOs.:109-135 are useful in specifically detecting PCR-amplification products of E1 cDNAs of HCV isolates belonging to one of the twelve HCV genotypes disclosed herein. In a preferred embodiment, probes having sequences substantially homologous to the sequences shown in SEQ ID NOs:109-135 are used alone or in combination with other probes specific to the same genotype.

For example, a probe having a sequence according to SEQ ID NO:109 can be used alone or in combination with a probe having a sequence according to SEQ ID NO:110. The probes derived from SEQ ID NOs:109-135 can range in size from about 30 to about 70 nucleotides and can be synthesized as described earlier.

The nucleic acid sequence used as a probe to detect PCR amplification products of the present invention can be labeled in single-stranded or double-stranded form. Labelling of the nucleic acid sequence can be carried out by techniques known to one skilled in the art. Such labelling techniques can include radiolabels and enzymes (Sambrook, J. et al. (1989) in "Molecular Cloning, A Laboratory Manual", Cold Spring Harbor Press, Plainview, N.Y.). In addition, there are known non-radioactive techniques for signal amplification including methods for attaching chemical moieties to pyrimidine and purine rings (Dale, R. N. K. et al. (1973) Proc. Natl. Acad. Sci., 70:2238-2242; Heck, R. F. (1968) S. Am. Chem. Soc., 90:5518-5523), methods which allow detection by chemiluminescence (Barton, S. K. et al. (1992) J. Am. Chem. Soc., 114:8736-8740) and methods utilizing biotinylated nucleic acid probes (Johnson, T. K. et al. (1983) Anal. Biochem., 133:126-131; Erickson, P. F. et al. (1982) J. of Immunology Methods, 51:241-249; Matthaei, F. S. et al. (1986) Anal. Biochem., 157:123-128) and methods which allow detection by fluorescence using commercially available products.

The present invention also relates to computer analysis of the amino acid sequences shown in SEQ ID NOs:52-102 by the program GENALIGN. This analysis groups the 51 amino acid sequences shown in SEQ ID NOs:52-102 into the twelve genotypes disclosed earlier in this application based upon the degree of variation of the amino acid sequences. For the purposes of the present invention, the amino acid sequence identity of E1 amino acid sequences of the same genotype ranges from about 85% to about 100% whereas the identity of E1 sequences of different genotypes ranges from about 45% to about 80%.

The grouping of SEQ ID NOs:52-102 into the twelve HCV genotypes is shown below:

    ______________________________________     SEQ ID NOs:     Genotypes     ______________________________________     52-59           I/1a     60-76           II/1b     77-80           III/2a     81-84           IV/2b     85              2c     86-90           V/3a     91              4a     92              4b     93-94           4c     95              4d      96-101         5a     102             6a     ______________________________________

For those genotypes containing more than one E1 amino acid sequence, computer alignment of the constituent sequences of each genotype was conducted using the computer program GENALIGN in order to produce a consensus sequence for each genotype. These alignments and their resultant consensus sequences are shown in FIGS. 2A-1 through 2G-2 for the seven genotypes (I/1a, II/1b, III/2a, IV/2b, V/3a, 4c and 5a) which comprise more than one sequence. Further alignment of the consensus sequences shown in FIGS. 2A-G with the amino acid sequences of SEQ ID NO:85 (genotype 2c); SEQ ID NO:91 (genotype 4a); SEQ ID NO:92 (genotype 4b); SEQ ID NO:95 (genotype 4d) and SEQ ID NO:102 (genotype 6a) to produce a consensus amino acid sequence for all twelve genotypes is shown in FIGS. 2H-1 and and 2H-2. The multiple alignment of E1 amino acid sequences shown in FIGS. 2A-H serves to highlight regions of homology and non-homology between amino acid sequences and hence, these alignments can readily be used by one skilled in the art to derive peptides useful in assays and vaccines for the diagnosis and prevention of HCV infection. Examples of purified and isolated peptides are provided by the present invention are shown as SEQ ID NOs:136-159. These peptides are derived from two regions of the amino acid sequences shown in FIGS. 2A-1 through 2H-2, amino acids 48-80 and amino acids 138-160. The peptides shown in SEQ ID NOs. 136-159 are useful as genotype-specific diagnostic reagents since they are capable of detecting an immune response specific to HCV isolates belonging to a single genotype. The genotype-specificity of the peptides shown in SEQ ID NOs:136-159 are as follows: SEQ ID NOs:136 and 148 are specific for genotype IV/2b; SEQ ID NOs:137 and 149 are specific for genotype 2c; SEQ ID NOs:138 and 150 are specific for genotype III/2a; SEQ ID NOs:139 and 151 are specific for genotype V/a; SEQ ID NOs:140 and 152 are specific for genotype II/1b; SEQ ID NOs:141 and 153 are specific for genotype I/1a; SEQ ID NOs:142 and 154 are specific for genotype 4a; SEQ ID NOs:143 and 155 are specific for genotype 4c; SEQ ID NOs:144 and 156 are specific for genotype 4d; SEQ ID NOs:145 and 157 are specific for genotype 4b; SEQ ID NOs:146 and 158 are specific for genotype 5a and SEQ ID NOs:147 and 159 are specific for genotype 6a. In SEQ ID NO:136, Xaa at position 22 is a residue of Ala or Thr, Xaa at position 24 is a residue of Val or Ile, Xaa at position 26 is a residue of Val or Met; in SEQ ID NO:138, Xaa at position 5 is a Ser or Thr residue, Xaa at position 11 is an Arg or Gln residue, Xaa at position 12 is an Arg or Gln residue; in SEQ ID NO:139, Xaa at position 3 is a Pro or Ser residue, Xaa at position 33 is a Leu or Met residue; in SEQ ID NO:140, Xaa at position 5 is a Thr or Ala residue, Xaa at position 13 is a Gly, Ala, Ser, Val or Thr residue, Xaa at position 14 is a Ser, Thr or Asn residue, Xaa at position 15 is a Val or Ile residue, Xaa at position 16 is a Pro or Ser residue, Xaa at position 18 is a Thr or Lys residue, Xaa at position 19 is a Thr or Ala residue, Xaa at position 22 is an Arg or His residue, Xaa at position 32 is an Ala, Val or Thr residue; in SEQ ID NO:141, Xaa at position 3 is an Ala or Pro residue, Xaa at position 4 is a Val or Met residue, Xaa at position 5 is a Thr or Ala residue, Xaa at position 17 is a Thr or Ala residue, Xaa at position 18 is a Thr or Ala residue, Xaa at position 23 is a His or Tyr residue; in SEQ ID NO:143, Xaa at position 10 is a Val or Ala residue, Xaa at position 11 is a Ser or Pro residue, Xaa at position 18 is an Asp or Glu residue Xaa at position 20 is a Leu or Ile residue; in SEQ ID NO:146, Xaa at position 3 is a Gln or His residue, Xaa at position 12 is an Asn, Ser or Thr residue, Xaa at position 13 is a Leu or Phe residue, Xaa at position 23 is an Ala or Val residue; in SEQ ID NO:148, Xaa at position 16 is a Val or Ala residue, Xaa at position 18 is a Glu or Gln residue; in SEQ ID NO:150, Xaa at position 2 is an Ala or Thr residue, Xaa at position 4 is a Met or Leu residue, Xaa at position 9 is an Ala or Val residue, Xaa at position 17 is an Ile or Leu residue, Xaa at position 20 is an Ile or Val residue, Xaa at position 21 is a Ser or Gly residue; in SEQ ID NO:151, Xaa at position 9 is a Val or Ile residue, Xaa at position 16 is a Leu or Val residue, Xaa at position 20 is an Ile or Leu residue; in SEQ ID NO:152, Xaa at position 2 is an Ala or Thr residue, Xaa at position 6 is a Val or Leu residue, Xaa at position 12 is an Ile or Leu residue, Xaa at position 16 is a Val or Ile residue, Xaa at position 17 is a Val, Leu or Met residue, Xaa at position 19 is a Met or Val residue, Xaa at position 21 is an Ala or Thr residue; in SEQ ID NO:153, Xaa at position 2 is a Thr or Ala residue, Xaa at position 6 is a Val, Ile or Met residue, Xaa at position 12 is an Ile or Val residue, Xaa at position 16 is a Ile or Val residue; in SEQ ID NO:155, Xaa at position 5 is a Leu or Val residue, Xaa at position 21 is a Thr or Ala residue; in SEQ ID NO:158, Xaa at position 1 is a Thr or Ala residue, Xaa at position 5 is a Val or Leu residue, Xaa at position 9 is a Leu, Met or Val residue, Xaa at position 23 is a Gly or Ala residue.

Those skilled in the art would be aware that the peptides of the present invention or analogs thereof can be synthesized by automated instruments sold by a variety of manufacturers or can be commercially custom-ordered and prepared. The term analog has been described earlier in the specification and for purposes of describing the peptides of the present invention, analogs can further include branched or non-linear arrangements of the peptide sequences shown in SEQ ID NOs:136-159.

Alternatively, peptides can be expressed from nucleic acid sequences where such sequences can be DNA, cDNA, RNA or any variant thereof which is capable of directing protein synthesis. In one embodiment, restriction digest fragments containing a coding sequence for a peptide can be inserted into a suitable expression vector that functions in prokaryotic or eukaryotic cells. Such restriction digest fragments may be obtained from clones isolated from prokaryotic or eukaryotic sources which encode the peptide sequence.

Suitable expression vectors and methods of isolating clones encoding the peptide sequences of the present invention have previously been described.

The preferred size of the peptides of the present invention is from about 8 to about 100 amino acids in length.

The present invention further relates to the use of the peptides shown in SEQ ID NOs:136-159 in methods of detecting antibodies specific for HCV in biological samples. In one embodiment, at least one peptide specific for a single genotype to be used in previously described immunoassays to detect antibodies specific for a single genotype of HCV. A preferred immunoassay is ELISA.

It is understood by one skilled in the art that the diagnostic assays described herein using genotype-specific oligonucleotides or genotype-specific peptides ca be useful in assisting one skilled in the art to choose a course of therapy for the HCV-infected individual.

In an alternative embodiment, a mixture of peptides can be used in an immunoassay to detect antibodies to any of the twelve genotypes of HCV. The mixture of peptides as disclosed herein, comprises at least one peptide selected from SEQ ID NOs:140-141 and 152-153; one peptide selected from SEQ ID NOs:136, 138, 148 and 150; one peptide selected from SEQ ID NOs:142-145 and 154-157; one peptide selected from SEQ ID NOs:146 and 158; one peptide selected from SEQ ID NOs:139 and 151; one peptide selected from SEQ ID NOs:138 and 150 and one peptide selected from SEQ ID NOs:140 and 159. In a preferred embodiment, the peptides of the present invention can be used in an ELISA assay as described previously for E1 proteins.

The peptides or analogs thereof may be prepared in the form of a kit, alone or in combinations with other reagents such as secondary antibodies, for use in immunoassay. In addition, since genotype-specific peptides shown in SEQ ID NOs:136-159 are derived from two variable regions in the E1 protein, amino acids 48-80 (SEQ ID NOs:136-147) and amino acids 138-160 (SEQ ID NOs:148-159), one skilled in the art would recognize that these peptides would be useful as vaccines against hepatitis C. In the present invention, a peptide from SEQ ID NOs:136-159 can be used alone or in combination with other peptides shown therein as immunogens in the vaccine. Formulations suitable for administering the peptide(s) of the present invention, routes of administration, pharmaceutical compositions comprising the peptides and so forth are the same as those previously described for recombinant E1 proteins. In addition, as described for E1 proteins, the peptide(s) can also be used to prepare antibodies to HCV-E1 protein.

The peptides of the present invention may also be supplied in the form of a kit, alone, or in the form of a pharmaceutical composition as described above for E1 proteins recombinant.

Any articles or patents referenced herein are incorporated by reference. The following examples illustrate various aspects of the invention but are in no way intended to limit the scope thereof.

MATERIALS

Serum used in these examples was obtained from 84 anti-HCV positive individuals that were previously found to be positive for HCV RNA in a cDNA PCR assay with primer set a from the 5' NC region of the HCV genome (Bukh, J. et al. (1992 (b)) Natl. Acad. Sci. USA 89:4942-4946). These samples were from 12 countries: Denmark (DK); Dominican Republic (DR); Germany (D); Hong Kong (HK); India (IND); Sardinia, Italy (S) ; Peru (P) ; South Africa (SA); Sweden (SW); Taiwan (T); United States (US); and Zaire (Z) .

EXAMPLE 1

Identification of the DNA Sequence of the E1 Gene of 51 Isolates of HCV via RT-PCR Analysis of Viral RNA Using Universal Primers

Viral RNA was extracted from 100 μl of serum by the guanidinium-phenol-chloroform method and the final RNA solution was divided into 10 equal aliquots and stored at -80° C. as described (Bukh, et al. (1992 (a)). The sequences of the synthetic oligonucleotides used in the RT-PCR assay, deduced from the sequence of HCV strain H-77 (Ogata, N. et al. (1991) Proc. Natl. Acad. Sci. USA 88:3392-3396), are shown as SEQ ID NOs:103-108. One aliquot of the final RNA solution, equivalent to 10 μl of serum, was used for cDNA synthesis that was performed in a 20 μl reaction mixture using avian myeloblastosis virus reverse transcriptase (Promega, Madison, Wis.) and SEQ ID NO:104 as a primer. The resulting cDNA was amplified in a "nested" PCR assay by Taq DNA polymerase (Amplitaq, Perkin-Elmer/Cetus) as described previously (Bukh et al. (1992a)) with primer set e (SEQ ID NOs:103-106). Precautions were taken to avoid contamination with exogenous HCV nucleic acid (Bukh et al. 1992a)), and negative controls (normal, uninfected serum) were interspersed between every test sample in both the RNA extraction and cDNA PCR procedures. No false positive results were observed in the analysis. In most instances, amplified DNA (first or second PCR products) was reamplified with primers SEQ ID NO:107 and SEQ ID NO:108 prior to sequencing since these two primers contained EcoR1 sites which would facilitate future cloning of the E1 gene. Amplified DNA was purified by gel electrophoresis followed by glass-milk extraction (Geneclean, BIO 101, LaJolla, Calif.) and both strands were sequenced directly by the dideoxy-nucleotide chain termination method (Bachman, B. et al. (1990) Nucl. Acids Res. 18:1309)) with phage T7 DNA polymerase (Sequenase, United States Biochemicals, Cleveland, Ohio), alpha ³³ S!dATP (Amersham, Arlington Heights, Ill.) or alpha ³³ P! dATP (Amersham or DuPont, Wilmington, Del.) and sequencing primers. RNA extracted from serum containing HCV strain H-77, previously sequenced by Ogata, N. et al. (1991), was amplified with primer set e (SEQ ID NOs:103-106) and sequenced in parallel as a control. The nucleotide sequences of the envelope 1 (E1) gene of all 51 HCV isolates are shown as SEQ ID NOs:1-51. In all 51 HCV isolates, the E1 gene was exactly 576 nucleotides in length and did not have any in-frame stop codons.

EXAMPLE 2

Computer Analysis of the Nucleotide and Deduced Amino Acid Sequences of the E1 Gene of the 51 HCV Isolates

Multiple computer-generated alignments of the nucleotide (SEQ ID NOs:1-51, FIGS. 1A-1 through 2H-2) and deduced amino acid sequences (SEQ ID NOs:52-102, FIGS. 2A-1 through 2H-2) of the cDNAs of the 51 HCV isolates constructed using the computer program GENALIGN (Miller, R. H. et al. (1990) Proc. Natl. Acad. Sci. USA 87:2057-2061) resulted in the 51 HCV isolates being divided into twelve genotypes based upon the degree of variation of the E1 gene sequence as shown in table 1.

                                      TABLE 1     __________________________________________________________________________     Biochemistry: Bukh et al.     Percent nucleotide (nt) and amino acid (aa) sequence identify of the E1     gene among the 12 HCV genotypes.            II/     I/1a   1b III/2a                   IV/2b                        2c   (V)/3a                                  4a   4b   4c   4d   5a   6a   nt:     __________________________________________________________________________         89.9-            72.0-               59.2-                   56.1-58.3                        60.8-62.8                             63.0-66.3                                  63.9-67.2                                       64.9-66.8                                            62.7-64.4                                                 67.7-69.4                                                      62.3-67.2                                                           62.2-63.9                                                                I/1a         97.6            76.2               63.7     aa:    88.9-               58.3-                   53.8-57.5                        60.1-61.5                             63.9-67.2                                  60.9-63.7                                       63.4-65.8                                            61.6-65.1                                                 63.0-65.5                                                      62.2-66.5                                                           61.6-63.0                                                                II/1b            97.9               62.2     I/1a         91.1- 88.0-                   69.1-71.0                        72.7-73.6                             58.0-60.8                                  61.5-62.7                                       59.9-60.4                                            59.7-63.4                                                 58.7-61.3                                                      56.6-60.8                                                           55.0-56.8                                                                III/2a         98.4  91.3     II/1b         75.5-            90.1-  92.7-95.0                        67.5-68.9                             56.3-58.3                                  58.9-60.8                                       56.4-57.6                                            57.1-59.9                                                 57.5-59.0                                                      53.5-56.6                                                           53.6-55.2                                                                IV/2b         80.7            97.9     III/2a         58.3-            52.6-               89.1-    --   57.5-58.2                                  59.2 58.5 58.0-58.3                                                 58.9 56.9-57.1                                                           57.6 2c         64.6            56.8               92.7     IV/2b         54.2-            51.0-               69.3-                   93.8-96.4 93.8-99.1                                  64.4-65.3                                       62.7-64.1                                            60.9-62.5                                                 62.3-63.9                                                      61.8-64.4                                                           58.0-58.9                                                                (V)/3a         56.8            54.2               72.9     2c  56.3-            52.6-               74.5-                   67.7-69.8                        --        --   74.8 75.5-78.0                                                 74.8 62.8-64.6                                                           62.0 4a         60.4            55.7               77.1     (V)/3a         64.1-            66.7-               54.7-                   54.2-56.8                        52.1-53.6                             94.3-98.4 --   74.0-74.8                                                 72.0 63.9-64.6                                                           62.7 4b         68.8            70.8               58.9     4a  69.3-            64.6-               62.0-                   58.9-60.4                        58.3 66.1-68.8                                  --        90.1 77.6-78.6                                                      62.7-64.8                                                           63.0-64.4                                                                4c         73.4            67.2               63.0     4b  66.7-            66.1-               53.6-                   52.1-53.1                        53.6 62.0-64.6                                  76.0 --        --   64.4-66.1                                                           64.1 4d         69.3            70.3               56.3     4c  66.1-            64.6-               55.2-                   54.2-58.3                        54.7-58.3                             63.0-65.6                                  77.1-81.3                                       79.2-80.2                                            89.6      90.1-95.7                                                           60.6-63.2                                                                5a         72.9            69.3               61.5     4d  73.4-            66.7-               56.3-                   55.2-55.7                        54.2 63.5-64.6                                  78.1 77.6 82.8 --        --   6a         75.5            70.3               58.9     5a  66.1-            64.1-               52.6-                   50.5-53.1                        54.2-56.3                             60.4-64.1                                  67.2-68.2                                       65.1-67.2                                            67.7-71.4                                                 69.3-71                                                      92.7-97.4         73.4            70.3               57.3     6a  64.6-            62.5-               49.0-                   49.0-50.5                        50.5 57.8-58.9                                  66.1 62.5 66.1-67.2                                                 66.7 62.0-63.5                                                           --         65.6            65.6               51.0     __________________________________________________________________________      Nucleotide sequences analyzed in compiling the above table are shown SEQ      ID NOs: 1-51 while the amino acid sequences analyzed are shown in SEQ ID      NOs: 52-102. The grouping of SEQ ID NOs: into genotypes is previously      described in the specification.

The nucleotide and amino acid sequence identity of HCV isolates of the same genotype was in the range of 88.0-99.1% and 89.1-98.4%, respectively, whereas that of HCV isolates of different genotypes was in the range of 53.5-78.6% and 49.0-82.8%, respectively. The latter differences are similar to those found when comparing the envelope gene sequences of the various serotypes of the related flaviviruses, as well as other RNA viruses. When microheterogenicity in a sequence was observed, defined as more than one prominent nucleotide at a specific position, the nucleotide that was identical to that of the HCV prototype (HCV1, Choo et al. (1989)) was reported if possible. Alternatively, the nucleotide that was identical to the most closely related isolate is shown.

Analysis of the consensus sequence of the E1 protein of the 51 HCV isolates from this study demonstrated that a total of 60 (30.3%) of the 192 amino acids of the E1 protein were invariant among these isolates (FIGS. 3A and 3B). Most impressive, all 8 cysteine residues as well as 6 of 8 proline residues were invariant. The most abundant amino acids (e.g. alanine, valine and leucine) showed a very low degree of conservation. The consensus sequence of the E1 protein contained 5 potential N-linked glycosylation sites. Three sites at positions 209, 305 and 325 were maintained in all 51 HCV isolates. A site at position 196 was maintained in all isolates except the sole isolate of genotype 2c. Also, a site at position 234 was maintained in all isolates except one isolate of genotype I/1a, all four isolates of genotype IV/2b and the sole isolate of genotype 6a. Conversely, only genotype IV/2b isolates had a potential glycosylation site at position 233. Further analysis revealed a highly conserved amino acid domain (aa 302-328) in the E1 protein with 20 (74.1%) of 27 amino acids invariant among all 51 HCV isolates. It is possible that the 5' and 3' ends of this domain are conserved due to important cysteine residues and N-linked glycosylation sites. The central sequence, 5'-GHRMAWDMM-3' (aa 315-323), may be conserved due to additional functional constraints on the protein structure. Finally, although the amino acid sequence surrounding the putative E1 protein cleavage site was variable, an amino acid doublet (GV) at position 380 was invariant among all HCV isolates.

A dendrogram of the genetic relatedness of the E1 protein of selected HCV isolates representing the 12 genotypes is shown in FIG. 4. This dendrogram was constructed using the program CLUSTAL (Weiner, A. J. et al. (1991) Virology 180:842-848) and had a limit of 25 sequences. The scale showing percent identity was added based upon manual calculation. From the 51 HCV isolates for which the complete sequence of the E1 gene region was obtained, 25 isolates representing the twelve genotypes were selected for analysis as follows. Among isolates with genotype I/1a (SEQ ID NOs:52-59), as well as among isolates with genotype II/1b (SEQ ID NOs:60-76) the two isolates with the lowest amino acid identity within each genotype were included. Among isolates of genotype IV/2b, isolate DK8 (SEQ ID NO:81) that has an amino acid identity of 96.4% to isolate T8 (SEQ ID NO:84) was excluded. Among isolates of genotype V/3a, isolates S2 (SEQ ID NO:88) and S54 (SEQ ID NO:90) that both shared 97.9% of the amino acids of isolates HK10 (SEQ ID NO:87) and S52 (SEQ ID NO:89) were excluded. Finally, among isolates of genotype VI, isolates SA4 (SEQ ID NO:97) and SA5 (SEQ ID NO:98) with an amino acid identity to isolate SA7 (SEQ ID NO:100) of 96.4% and 95.8%, respectively were excluded. This dendrogram in combination with the analysis of the E1 gene sequence of 51 HCV isolates in Table 1 demonstrates extensive heterogeneity of this important gene.

The worldwide distribution of the 12 genotypes among 74 HCV isolates is depicted in FIG. 5. The complete E1 gene sequence was determined in 51 of these HCV isolates (SEQ ID NOs:1-51), including 8 isolates of genotype I/1a, 17 isolates of genotype II/1b and 26 isolates comprising genotypes III/2a, IV/2b, 2c, 3a, 4a-4d, 5a and 6a. In the remaining 23 isolates, all of genotypes I/1a and II/1b, the genotype assignment was based on a partial E1 gene sequence since they did not represent additional genotypes in any of the 12 countries. The number of isolates of a particular genotype is given in each of the 12 countries studied. Of the twelve genotypes, genotypes I/1a and II/1b were the most common accounting for 48 (65%) of the 74 isolates. Analysis of the E1 gene sequences available in the GenBank data base at the time of this study revealed that all 44 such sequences were of genotypes I/1a, II/1b, III/2a and IV/2b. Thus, based upon E1 gene analysis, 8 new genotypes of HCV have been identified.

Also of interest, different HCV genotypes were frequently found in the same country, with the highest number of genotypes (five) being detected in Denmark. Of the twelve genotypes, genotypes I/1a, II/1b, III/2a, IV/2b and V/3a were widely distributed with genotype II/1b being identified in 11 of 12 countries studied (Zaire was the only exception). In addition, while genotypes I/1a and II/1b were predominant in the Americas, Europe and Asia, several new genotypes were predominant in Africa.

It was also found that genotypes I/1a, II/1b, III/2a, IV/2b and V/3a of HCV were widely distributed around the world, whereas genotypes 2c, 4a, 4b, 4d, 5a and 6a were identified only in discreet geographical regions. For example, the majority of isolates in South Africa comprised a new genotype (5a) and all isolates in Zaire comprised 3 new closely related genotypes (4a, 4b, 4c). These genotypes were not identified outside Africa.

EXAMPLE 3

Detection by ELISA Based on Antigen from Insect Cells Expressing Complete E1 Protein

Expression of E1 protein in SF9 cells. A cDNA (SEQ ID NO:1) encoding the complete E1 protein of SEQ ID NO:52 is subcloned into pBlueBac--Transfer vector (Invitrogen) using standard subcloning procedures. The resultant recombinant expression vector is cotransfected into SF9 insect cells (Invitrogen) by the Ca precipitation method according to the Invitrogen protocol.

ELISA Based on Infected SF9 cells. 5×10⁶ SF9 cells infected with the above-described recombinant expression vector are resuspended in 1 ml of 10 mM Tris-HCl, pH 7.5, 0.15M NaCl and are then frozen and thawed 3 times. 10 ul of this suspension is dissolved in 10 ml of carbonate buffer (pH 9.6) and used to cover one flexible microtiter assay plate (Falcon). Serum samples are diluted 1:20, 1:400 and 1:8000, or 1:100, 1:1000 and 1:10000. Blocking and washing solutions for use in the ELISA assay are PBS containing 10% fetal calf serum and 0.5% gelatin (blocking solution) and PBS with 0.05% Tween -20 (Sigma, St.Louis, Mo.) (washing solution). As a secondary antibody, peroxidase-conjugated goat IgG fraction to human IgG or horse radish peroxidase-labelled goat anti-Old or anti-New World monkey immunoglobulin is used. The results are determined by measuring the optical density (O.D.) at 405 nm.

To determine if insect cells-derived E1 protein representing genotype I/a of HCV could detect anti-HCV antibody in chimpanzees infected with genotype I/1a of HCV, three infected chimpanzees are examined. The serum of all 3 chimpanzees are found to seroconvert to anti-HCV.

EXAMPLE 4

Use of the Complete E1 Protein as a Vaccine

Mammals are immunized with purified or partially purified E1 protein in an amount sufficient to stimulate the production of protective antibodies. The immunized mammals challenged with various genotypes of HCV are protected.

It is understood by one skilled in the art that the recombinant E1 protein used in the above vaccine can also be used in combination with other recombinant E1 proteins having an amino acid sequence shown in SEQ ID NOs:52-102.

EXAMPLE 5

Determination of the Genotype of an HCV Isolate Via Hybridization of Genotype-Specific Oligonucleotides to RT-PCR Amplification Products.

Viral RNA is isolated from serum obtained from a mammal and is subjected to RT-PCR as in Example 1. Following amplification, the amplified DNA is purified as described in Example 1 and aliquots of 100 mg of amplification product are applied to twelve dots on a nitrocellulose filter set in a dot blot apparatus. The twelve dots are then cut into separate dots and each dot is hybridized to a ³² p-labelled oligonucleotide specific for a single genotype of HCV. The oligonucleotides to be used as hybridization probes are selected from SEQ ID NOs:109-135.

EXAMPLE 6

ELISA Based on Synthetic Peptides Derived From E1 cDNA Sequences

Synthetic peptides specific for genotype I/1a and having amino acid sequences according to SEQ ID NOs:136-148 are placed in 0.1% PBS buffer and 50 ul of 1 mg/ml of peptide is used to cover each well of the microtiter assay plate. Serum samples from two mammals infected with genotype I/1a HCV and from one mammal infected with genotype 5a HCV are diluted as in Example 3 and the ELISA is carried out as in Example 3. Both mammals infected with genotype I HCV react positively with peptides while the mammal infected with genotype 5a HCV exhibit no reactivity.

EXAMPLE 7

Use of the E1 Peptides as a Vaccine

Since the E1 genotype-specific peptides of the present invention are derived from two variable regions in the complete E1 protein, there exists support for the use of these peptides as a vaccine to protect against a variety of HCV genotypes. Mammals are immunized with peptide(s) selected from SEQ ID NOs: 136-159 in an amount sufficient to stimulate production of protective antibodies. The immunized mammals challenged with various genotypes of HCV are protected.

    __________________________________________________________________________     SEQUENCE LISTING     (1) GENERAL INFORMATION:     (iii) NUMBER OF SEQUENCES: 159     (2) INFORMATION FOR SEQ ID NO:1:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK7     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:     TACCAAGTGCGCAACTCCACGGGGCTTTACCATGTCACC39     AATGATTGCCCTAACTCGAGTATCGTGTACGAGGCGGCC78     GATGCCATCCTGCACACTCCGGGGTGTGTCCCTTGCGTT117     CGCGAGGGTAACGTCTCGAGGTGTTGGGTGGCGATGACC156     CCCACGGTGGCCACCAGGGATGGCAAACTCCCCACAGCG195     CAGCTTCGACGTCACATCGATCTGCTCGTCGGGAGTGCC234     ACCCTCTGTTCGGCCCTCTACGTGGGGGACCTGTGCGGG273     TCTGTCTTTCTTGTCGGTCAACTGTTTACCTTCTCTCCC312     AGGCGCCACTGGACGACGCAAGGCTGCAATTGTTCTATC351     TATCCTGGCCATATAACGGGTCACCGCATGGCGTGGGAT390     ATGATGATGAACTGGTCCCCTACCACGGCGTTGGTAGTA429     GCTCAGCTGCTCCGGATCCCGCAAGCCATCTTGGACATG468     ATCGCTGGTGCTCACTGGGGAGTCCTGGCGGGCATAGCG507     TATTTTTCCATGGTGGGGAACTGGGCGAAGGTCCTGGTA546     GTGCTGCTGCTATTTGCCGGCGTCGACGCG576     (2) INFORMATION FOR SEQ ID NO:2:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK9     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:     TACCAAGTACGCAACTCCTCGGGCCTCTACCATGTCACC39     AATGATTGCCCTAACTCGAGTATTGTGTACGAGGCGGCC78     GATGCCATCCTGCATTCTCCAGGGTGTGTCCCTTGCGTT117     CGCGAGGGTAACGCCTCGAAATGTTGGGTGGCGGTGGCC156     CCCACGGTGGCCACCAGGGACGGCAAGCTCCCCGCAACG195     CAGCTTCGACGTCACATCGATCTGCTTGTCGGGAGCGCC234     ACCCTCTGCTCGGCCCTCTATGTGGGGGACTTGTGCGGG273     TCTGTCTTCCTTGTCGGCCAACTGTTCACCTTCTCCCCC312     AGACGCCACTGGACAACGCAAGACTGCAACTGTTCTATC351     TACCCCGGCCATATTACGGGTCATCGCATGGCGTGGGAT390     ATGATGATGAACTGGTCCCCTACAGCAGCGCTGGTAATG429     GCGCAGCTGCTCAGGATCCCGCAGGCCATCTTGGACATG468     ATCGCTGGTGCCCACTGGGGAGTCCTAGCGGGCATAGCG507     TATTTCTCCATGGTGGGGAACTGGGCGAAGGTCGTGGTG546     GTACTGTTGCTGTTTACCGGCGTCGATGCG576     (2) INFORMATION FOR SEQ ID NO:3:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DR1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:     CACCAAGTGCGCAACTCTACAGGGCTTTACCATGTCACC39     AATGATTGCCCTAATTCGAGTATTGTGTACGAGGCGGCC78     GATGCCATCCTGCACGCGCCGGGGTGTGTCCCTTGCGTT117     CGCGAGGGTAACGCCTCGAGGTGTTGGGTGGCGGTGACC156     CCCACGGTGGCCACCAGGGACGGCAAACTCCCCACAACG195     CAGCTTCGACGTCACATCGACCTGCTTGTCGGGAGCGCC234     ACCCTCTGCTCGGCCCTCTACGTGGGGGACCTGTGCGGG273     TCTGTCTTCCTTGTCGGTCAACTGTTCACCTTTTCTCCC312     AGGCGCCACTGGACAACGCAAGACTGCAATTGTTCTATC351     TATCCCGGCCATATAACGGGACACCGTATGGCATGGGAT390     ATGATGATGAACTGGTCCCCTACGACAGCGCTGGTAATG429     GCTCAGCTGCTCCGGATCCCACAAGCCATCTTGGACATG468     ATCGCTGGAGCCCACTGGGGAGTCCTAGCGGGCATAGCG507     TATTTCTCCATGGTGGGGAACTGGGCGAAGGTCGTGGTA546     GTGCTGTTGCTGTTTGCCGGCGTTGATGCG576     (2) INFORMATION FOR SEQ ID NO:4:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DR4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:     CACCAAGTGCGCAACTCTACAGGGCTTTACCATGTCACC39     AATGATTGCCCTAATTCGAGTATTGTGTACGAGGCGGCC78     GATGCCATCCTGCACACGCCGGGGTGTGTCCCTTGCGTT117     CGCGAGGGTAACACCTCGAGGTGTTGGGTGGCGGTGACC156     CCCACGGTGGCCACCAGGGACGGCAAACTCCCCACAACG195     CAGCTCCGACGTCACATCGACCTGCTTGTCGGGAGCGCC234     ACCCTCTGCTCGGCCCTCTACGTGGGGGACTTGTGCGGG273     TCTGTCTTCCTTGTCGGTCAACTGTTCACCTTCTCTCCC312     AGGCACCACTGGACAACGCAAGACTGCAATTGTTCCATC351     TATCCCGGCCATATAACGGGCCACCGCATGGCGTGGGAT390     ATGATGATGAACTGGTCCCCTACGACAGCGCTGGTAGTA429     GCTCAGCTGCTCCGGATCCCACAAGCCATCTTGGACATG468     ATCGCTGGTGCCCACTGGGGAGTCCTAGCGGGCATAGCG507     TATTTCTCCATGGTGGGGAACTGGGCGAAGGTCCTGGTA546     GTGCTGTTGCTGTTTGCCGGCGTTGATGCG576     (2) INFORMATION FOR SEQ ID NO:5:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S14     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:     TACCAAGTGCGCAACTCCACGGGGCTTTACCATGTTACC39     AATGATTGCCCTAACTCGAGTATTGTGTACGAGACAGCT78     GATGCTATCCTACACGCTCCGGGATGTGTCCCTTGCGTT117     CGTGAGGGTAACACCTCGAGGTGTTGGGTGGCGATGACC156     CCCACGGTGGCCACCAGGGACGGCAAACTCCCCGCAACG195     CAGCTTCGACGTTACATCGATCTGCTTGTCGGGAGCGCC234     ACCCTCTGTTCGGCCCTCTACGTGGGGGACTTGTGCGGG273     TCTGTCTTTCTTGTCGGTCAGCTGTTTACCTTCTCTCCC312     AGGCGCCTCTGGACGACGCAAGACTGCAATTGTTCTATC351     TATCCCGGCCATATAACGGGTCATCGCATGGCATGGGAT390     ATGATGATGAACTGGTCCCCTACGACGGCACTGGTAGTA429     GCTCAGCTGCTCCGGATCCCACAAGCCATCTTGGATATG468     ATCGCTGGTGCTCACTGGGGAGTCCTAGCGGGCATAGCG507     TATTTCTCCATGGTGGGAAACTGGGCGAAGGTCCTAGTG546     GTGCTGCTGCTATTCGCCGGCGTTGACGCG576     (2) INFORMATION FOR SEQ ID NO:6:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S18     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:     TACCAAGTACGCAACTCCACGGGCCTTTACCATGTCACC39     AATGACTGCCCTAACTCGAGCATTGTGTACGAGACGGCC78     GATACCATCCTACACTCTCCGGGGTGTGTCCCTTGCGTT117     CGCGAGGGTAACGCCTCGAGATGTTGGGTGCCGGTGGCC156     CCCACAGTTGCCACCAGGGACGGCAAACTCCCCGCAACG195     CAGCTTCGACGTCACATCGATCTGCTTGTTGGGAGCGCC234     ACCCTCTGCTCGGCCCTCTATGTGGGGGACCTGTGCGGG273     TCTGTCTTTCTTGTCAGCCAGCTGTTCACTATCTCCCCC312     AGGCGCCACTGGACAACGCAAGACTGCAACTGTTCTATC351     TACCCCGGCCATATAACGGGTCACCGTATGGCATGGGAT390     ATGATGATGAACTGGTCCCCTACAACGGCGTTGGTAATA429     GCTCAGCTGCTCAGGGTCCCGCAAGCCGTCTTGGACATG468     ATCGCTGGTGCCCACTGGGGAGTCCTAGCGGGCATAGCG507     TATTTCTCCATGGCGGGGAACTGGGCGAAGGTCCTGCTA546     GTGCTGTTGCTGTTTGCCGGCGTCGATGCG576     (2) INFORMATION FOR SEQ ID NO:7:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SW1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:     TACCAAGTACGCAACTCCTCGGGCCTTTACCATGTCACC39     AATGATTGCCCTAACTCGAGTATTGTGTACGAGACGGCC78     GATGCCATTCTACACTCTCCAGGGTGTGTCCCTTGCGTT117     CGCGAGGATGGCGCCCCGAAGTGTTGGGTGGCGGTGGCC156     CCCACAGTCGCCACTAGGGACGGCAAACTCCCTGCAACG195     CAGCTTCGACGTCACATCGATCTGCTTGTCGGAAGCGCC234     ACCCTCTGCTCGGCCCTCTACGTGGGGGACTTGTGCGGG273     TCTGTCTTTCTCGTCAGTCAACTGTTCACGTTCTCCCCC312     AGGCGCCACTGGACAACGCAAGACTGTAACTGTTCTATC351     TATCCCGGCCACATAACGGGTCACCGCATGGCATGGGAT390     ATGATGATGAACTGGTCCCCCACAACAGCGCTGGTAGTA429     GCTCAGCTGCTCAGGATCCCGCAAGCCGTCTTGGACATG468     ATCGCTGGTGCCCACTGGGGAGTCCTAGCGGGCATAGCG507     TATTTCTCCATGGTGGGGAACTGGGCGAAGGTCCTGATA546     GTGCTGTTGCTGTTTTCCGGCGTCGATGCG576     (2) INFORMATION FOR SEQ ID NO:8:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: US11     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:     TACCAAGTACGCAACTCCACGGGGCTTTACCATGTCACC39     AATGATTGCCCTAACTCGAGTATTGTGTACGAGGCGGCC78     GATGCCATCCTGCACACTCCGGGGTGTGTTCCTTGCGTT117     CGCGAGGGTAACGCTTCGAGGTGTTGGGTGGCGATGACC156     CCCACGGTGGCCACCAGGGACGGCAAACTCCCCACAACG195     CAACTTCGACGTCACATCGATCTGCTTGTCGGGAGCGCC234     ACCCTCTGTTCGGCCCTCTACGTGGGGGACCTGTGCGGG273     TCTGTCTTTCTTGTCGGTCAACTGTTTACCTTCTCTCCC312     AGACGCCACTGGACGACGCAGGGCTGCAATTGTTCTATC351     TATCCCGGCCATATAACGGGTCACCGCATGGCATGGGAT390     ATGATGATGAACTGGTCCCCTACGGCGGCGTTGGTGGTA429     GCTCAGCTGCTCCGGATCCCACAAGCCATCTTGGACATG468     ATCGCTGGTGCTCACTGGGGAGTCCTAGCGGGCATAGCG507     TATTTCTCCATGGTGGGGAACTGGGCGAAGGTCCTGGTA546     GTGCTGCTGCTATTTGCCGGCGTCGACGCG576     (2) INFORMATION FOR SEQ ID NO:9:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: D1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:     TATGAAGTGCGCAACGTGTCCGGGGTGTACCATGTCACG39     AACGACTGTTCCAACTCGAGCATTGTGTATGAGACAGCG78     GACATGATCATGCACACCCCCGGGTGCGTGCCCTGCGTT117     CGGGAGGACAACTCCTCTCGCTGCTGGGTAGCGCTCACC156     CCCACGCTCGCGGCTAGGAATGGCAACGTCCCCACTACG195     GCGATACGACGCCACGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCCATGTACGTGGGGGATCTCTGCGGA273     TCTGTTTTCCTCATCTCCCAGCTGTTCACCCTCTCGCCT312     CGCCGGCATGAGACGGTACAGGAGTGTAATTGCTCAATC351     TATCCCGGCCACGTGACAGGTCACCGTATGGCTTGGGAT390     ATGATGATGAACTGGTCACCTACAACAGCCTTAGTGGTA429     TCGCAGTTACTCCGGATCCCACAAGCTGTCATGGACATG468     GTGGCGGGGGCCCACTGGGGGGTCCTGGCGGGCCTCGCC507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTCTTTGCTGGCGTTGACGGC576     (2) INFORMATION FOR SEQ ID NO:10:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: D3     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:     TATGAAGTGCGCAACGTGTCCGGGGTGTACCAAGTCACC39     AATGACTGTTCCAACTCGAGCATCGTGTATGAGACAGCG78     GACATGATCATGCACACCCCCGGGTGCGTGCCCTGCGTT117     CGGGAGGACAACTCCTCTCGCTGCTGGGTAGCGCTCACC156     CCCACGCTCGCGGCTAGGAATAGCAGCGTCCCCACTACG195     ACAATACGACGCCACGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCCATGTACGTGGGGGATCTTTGCGGA273     TCTGTTTTCCTCGTCTCCCAGCTGTTCACCTTCTCGCCT312     CGCCGGCATGAGACAGTACAGGAATGTAACTGCTCAATC351     TATCCCGGCCACGTGACAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCGCCTACAGCAGCCCTAGTGGTA429     TCGCAGTTACTCCGGATCCCACAAGCTGTCGTGGACATG468     GTGGCGGGGGCCCACTGGGGGGTCCTGGCGGGCCTCGCC507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTCTTTGCTGGCGTCGACGGC576     (2) INFORMATION FOR SEQ ID NO:11:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:     TATGAAGTGCGCAACGTGTCCGGGGTGTACCACGTCACA39     AACGACTGCTCCAACTCAAGCATCGTGTATGAGGCAGTG78     GACGTGATCATGCATACCCCAGGGTGCGTGCCCTGCGTT117     CGGGAGAACAACCACTCCCGTTGCTGGGTAGCGCTCACC156     CCCACGCTCGCGGCCAGGAACGCCAGCATCCCCACTACG195     ACAATACGACGCCATGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCTATGTACGTGGGGGACCTCTGCGGA273     TCCGTTTTCCTCGTCTCTCAGCTGTTCACCTTTTCACCT312     CGCCGGCATGAGACAGCACAGGACTGCAACTGCTCAATC351     TATCCCGGCCACGTTTCAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCACCTACAACAGCCCTAGTGCTA429     TCGCAGTTACTCCGAATCCCACAAGCTGTCGTGGACATG468     GTGGCGGGGGCCCACTGGGGAGTCCTGGCGGGCCTCGCC507     TACTACTCCATGGCGGGGAACTGGGCCAAGGTTTTAATT546     GTGTTGCTACTCTTTGCCGGCGTTGATGGG576     (2) INFORMATION FOR SEQ ID NO:12:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK3     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:     TATGAAGTGCGCAACGTGTCCGGGATATACCATGTCACG39     AACGACTGCTCCAACTCAAGCGTCGTGTATGAGACAGCA78     GACATGATCATGCATACCCCTGGATGCGTGCCCTGCGTA117     CGGGAGAACAACTCCTCCCGCTGTTGGGTAGCGCTCACT156     CCCACGCTCGCGGCCAGGAACGTCAGCGTCCCCACCACG195     ACAATACGACGTCACGTCGACTTGCTCGTTGGGGCGGCT234     GCCTTCTGCTCCGCTATGTACGTGGGGGATCTCTGCGGA273     TCTGTTTTCCTTGTCTCCCAGCTGTTCACCTTCTCGCCT312     CGCCGACACGAGACAGTACAGGACTGCAACTGCTCACTC351     TATCCCGGCCACGTATCAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCCCCTACAGCAGCCCTAGTGGTG429     TCGCAATTACTCCGGATCCCGCAAGCTGTCGTGGACATG468     GTGGCGGGGGCCCACTGGGGAGTCCTAGCGGGCCTTGCC507     TACTATTCCATGGTGGGAAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTTTTTGCCGGCGTTGATGGG576     (2) INFORMATION FOR SEQ ID NO:13:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:     CATGAAGTGCACAACGTATCCGGGATCTACCATGTCACG39     AACGACTGCTCCAACTCAAGTATTGTGTATGAGGCAGCG78     GACATGATCATGCATACCCCCGGGTGCGTGCCCTGCGTC117     CGGGAGAACAACTCCTCCCGTTGCTGGGTAGCGCTCACT156     CCCACGCTCGCGGCCAGGAACGCCAGCATCCCCACTACG195     ACAATACGACGCCATGTCGACTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCCATGTACGTGGGAGATCTCTGCGGA273     TCTGTCTTCCTCGTCTCCCAGTTGTTCACCTTCTCGCCT312     CGCCGGCATGAGACGGTACAGGACTGCAATTGCTCAATC351     TATCCCGGCCACGTATCAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCACCTACAGCAGCCCTAGTGGTA429     TCGCAGTTACTCCGACTCCCACAAGCTGTCATGGACATG468     GTGGCGGGAGCCCACTGGGGAGTCCTAGCGGGCCTTGCT507     TACTATTCCATGGTGGGGAACTGGGCCAAGGTTTTGATT546     GTGATGCTACTCTTTGCCGGCGTTGACGGG576     (2) INFORMATION FOR SEQ ID NO:14:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK5     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:     TATGAAGTGCGCAACGTGTCCGGGGTATACCATGTCACG39     AACGACTGCTCCAACTTAAGCATCGTGTACGAGACAACG78     GACATGATCATGCACACCCCTGGGTGCGTGCCCTGCGTT117     CGGGAAAACAACTCCTCCCGTTGTTGGGTAGCGCTCGCC156     CCCACGCTCGCGGCCAGGAACGCCAGCGTCCCCACCACG195     GCAATACGACGCCACGTCGACTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCTATGTACGTGGGGGATCTTTGCGGA273     TCTGTTTTCCTCGTCTCCCAGCTGTTCACCTTCTCGCCT312     CGCCGACACGAGACGGTACAGGACTGCAACTGCTCAATC351     TATCCCGGCCACGTAACAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCACCTACAACAGCCCTAGTGGTG429     TCGCAGTTACTCCGGATCCCGCAAGCTGTCGTGGACATG468     GTAGCGGGGGCCCACTGGGGGGTCCTGGCGGGCCTTGCC507     TACTATTCCATGGTGGGAAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTTTTTGCCGGCGTTGATGGG576     (2) INFORMATION FOR SEQ ID NO:15:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK8     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:     TATGAAGTGCGCAACGTGTCCGGGATATACCATGTCACG39     AACGACTGCTCCAACTCAAGCATCGTGTATGAAACAGCG78     GACATGATTATGCATACCCCTGGATGCATGCCCTGCGTT117     CGGGAGAACAACTCCTCCCGTTGCTGGGTGGCGCTCACT156     CCCACGCTCGCGGCTAGGAATGTCAGCGTCCCCACTACG195     ACAATACGACGCCACGTCGACTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCTATGTACGTGGGGGATCTCTGCGGA273     TCTGTTTTCCTCGTCTCCCAGCTGTTCACCTTTTCGCCT312     CGCCGACACGAGACGGTACAGGACTGCAACTGCTCAATC351     TATCCCGGCCACGTATCAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCGCCCACAACAGCCCTAGTGGTG429     TCGCAGTTACTCCGGATCCCGCAAGCTATCGTGGACATG468     GTGGCGGGGGCCCACTGGGGAGTCCTAGCGGGCCTTGCC507     TACTATTCCATGGTGGGCAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTGTTTGCCGGCGTTGATGGG576     (2) INFORMATION FOR SEQ ID NO:16:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: IND5     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:     TATGAAGTGCGCAACGTGTCCGGGGTGTACCATGTCACG39     AACGACTGCTCCAACTCAAGTATTGTGTATGAGGCAGCG78     GACATGATCATGCACACTCCCGGGTGCGTGCCCTGCGTT117     CGGGAGGGCAACTCCTCTCGCTGCTGGGTAGCGCTCACT156     CCCACTCTCGCGGCCAGGAACGCCAGCGTCTCCACCACG195     ACAATACGACACCACGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGTTCCGCTATGTACGTGGGGGATCTATGCGGA273     TCTGTTTTCCTCGTCTCCCAGCTGTTCACCTTCTCACCG312     CGCCGGCATGAGACAGTACAGGACTGCAATTGCTCCATC351     TATCCCGGCCACGTATCAGGTCACCGCATGGCCTGGGAT390     ATGATGATGAACTGGTCACCTACAGCAGCCCTAGTGGTA429     TCGCAGTTGCTCCGGATCCCACAAGCTGTCGTGGATATG468     GTGGCGGGGGCCCACTGGGGAATCCTGGCGGGCCTTGCC507     TACTATTCCATGGTAGGGAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTCTTTGCCGGCGTTGACGGG576     (2) INFORMATION FOR SEQ ID NO:17:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: IND8     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:     TATGAGGTGCGCAACGTGTCCGGGGTGTACCATGTCACG39     AACGACTGCTCCAACTCAAGTATTGTGTATGAGGCAGCG78     GACATGATCATGCACACCCCCGGGTGCGTGCCCTGCGTT117     CGGGAGGGCAACTTCTCTAGTTGCTGGGTAGCGCTCACT156     CCCACTCTCGCGGCTAGGAACGCCAGCGTCCCCACCACG195     ACAATACGACGCCACGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGTTCCGCTATGTACGTGGGGGATCTCTGCGGA273     TCTGTTTTCCTTGTCTCCCAGCTGTTCACCTTCTCACCG312     CGCCGGCATGAGACAGTACAGGACTGCAATTGCTCCATC351     TATCCCGGCCACGTATCAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCACCTACAGCGGCCCTAGTGGTA429     TCGCAGTTGCTCCGGATCCCACAAGCTGTCGTGGATATG468     GTGGCGGGGGCCCACTGGGGAATCCTGGCGGGCCTTGCC507     TACTATTCCATGGTAGGGAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTCTTTGCCGGCGTTGACGGG576     (2) INFORMATION FOR SEQ ID NO:18:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: P10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:     TATGAAGTGCGCAACGTGTCCGGGGTGTACCATGTCACG39     AACGACTGCTCCAACTCAAGTATTGTGTATGAGGCAGCG78     GACATGATAATGCACACCCCCGGGTGCGTGCCCTGTGTT117     CGGGAGAACAACTCCTCCCGCTGCTGGGTAGCGCTCACT156     CCCACACTCGCGGCTAGGAATTCCAGCGTCCCAACTACG195     GCAATACGACGCCATGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCTATGTACGTGGGGGATCTCTGCGGA273     TCTGTTCTCCTCGTCTCCCAGCTGTTCACCTTCTCACCT312     CGCCGGCATTGGACAGTACAGGACTGCAATTGTTCAATC351     TATCCTGGCCACGTATCAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCGCCCACAGCAGCCCTAGTGGTG429     TCGCAGCTACTCCGGATCCCACAAGCTATCTTGGATGTG468     GTGGCGGGGGCCCACTGGGGAGTCCTGGCGGGCCTTGCC507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTCTTGATT546     GTGATGCTACTCTTTGCCGGCGTTGACGGA576     (2) INFORMATION FOR SEQ ID NO:19:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S9     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:     TATGAAGTGCGCAACGTATCCGGGGCGTACCATGTCACG39     AACGACTGCTCCAACTCAAGTATTGTGTACGAGGCAGCG78     GACGTGATCATGCATACCCCCGGGTGTGTACCCTGCGTT117     CAGGAGGGTAACTCCTCCCAATGCTGGGTGGCGCTCACC156     CCCACGCTCGCGGCCAGGAACGCTACCGTCCCCACCACG195     ACAATACGACGTCATGTCGATTTGCTCGTTGGGGCGGCT234     GTTTTCTGCTCCGCTATGTACGTGGGGGACCTGTGCGGA273     TCTGTTTTCCTCATCTCCCAGCTGTTCACCATCTCGCCC312     CGTCGGCATGAGACAGTACAGAACTGCAATTGCTCAATC351     TATCCCGGACACGTGACAGGTCATCGCATGGCCTGGGAT390     ATGATGATGAACTGGTCGCCTACAACAGCCCTAGTGGTA429     TCGCAGCTACTCCGGATCCCACAAGCTGTCATGGATATG468     GTGGCGGGGGCCCACTGGGGAGTCCTGGCGGGCCTCGCC507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTTTTTGCTGGTGTTGACGGG576     (2) INFORMATION FOR SEQ ID NO:20:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S45     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:     TATGAAGTGCGCAACGTGTCCGGGGCGTACCATGTCACG39     AACGACTGCTCCAACTCAAGCATTGTGTATGAGGCAGTG78     GACGTGATCCTGCACACCCCTGGGTGCGTGCCCTGCGTT117     CGGGAGAACAACTCCTCCCGTTGCTGGGTGGCGCTCACT156     CCCACGCTCGCGGCCAGGAACTCCAGCGTCCCCACTACG195     ACAATACGACGTCACGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCTATGTACGTGGGGGATCTCTGCGGA273     TCTGTTTTCCTTGTTTCCCAGCTGTTCACCTTCTCGCCT312     CGTCGGCATGAGACAGTACAGGACTGCAACTGTTCAATC351     TATCCCGGCCACGTAACAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCGCCTACAGCAGCCTTAGTGGTA429     TCGCAGTTACTCCGGATCCCACAAGCTGTCGTGGACATG468     GTGGCGGGGGCCCACTGGGGAGTCCTGGCGGGCCTTGCC507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTTCTGATT546     GTGATGCTACTCTTTGCCGGCGTTGACGGG576     (2) INFORMATION FOR SEQ ID NO:21:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:     TATGAAGTGCGCAACGTGTCCGGGATGTACCATGTCACG39     AACGACTGCTCCAACTCAAGCATTGTGTATGAGGCAGCG78     GACATGATCATGCACACCCCCGGGTGCGTGCCCTGCGTT117     CGGGAGAACAACTCCTCCCGCTGCTGGGTAGCGCTCACT156     CCCACGCTCGCGGCCAGGAACTCCAGCGTCCCCACTACG195     ACAATACGACGCCACGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCCATGTACGTGGGGGACCTCTGCGGA273     TCTGTTTTCCTTGTCTCCCAGCTGTTCACCTTCTCGCCT312     CGCCGGTATGAGACAGTACAGGACTGCAATTGCTCAATC351     TATCCCGGCCGCGTAACAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCACCTACAACAGCTCTAGTAGTA429     TCGCAGTTACTCCGGATCCCACAAGCTATCGTGGACATG468     GTGGCGGGGGCCCACTGGGGAGTCCTAGCGGGCCTTGCC507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTTTTGATT546     GTTATGCTACTCTTTGCCGGCGTTGACGGG576     (2) INFORMATION FOR SEQ ID NO:22:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SW2     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:     TATGAAGTGCGCAACGTGTCCGGGGTGTATCATGTCACG39     AACGACTGTTCCAACTCAAGCATTGTGTATGAGACAGCG78     GACATGATCATGCATACCCCCGGGTGCGTGCCCTGCGTT117     CGGGAGGCCAACTCCTCCCGCTGCTGGGTAGCGCTCACT156     CCCACGCTAGCAGCCAGGAACACCAGCGTCCCCACTACG195     ACAATACGACGCCACGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGTTATGTACGTGGGGGATCTCTGCGGA273     TCTGTTTTCCTCGTCTCCCAGCTGTTCACTTTTTCACCT312     CGCCGGCACGAGACAGTACAGGACTGCAACTGTTCCATC351     TATCCCGGCCACGTATCAGGTCACCGCATGGCTTGGGAC390     ATGATGATGAACTGGTCACCTACAGCAGCCCTGGTGGTA429     TCGCAGTTACTCCGGATCCCACAAGCTGTCGTGGACATG468     GTAGCGGGGGCCCACTGGGGAGTCCTGGCGGGCCTTGCA507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTTTTGATT546     GTGATGCTACTCTTTGCTGGCGTTGACGGG576     (2) INFORMATION FOR SEQ ID NO:23:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T3     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:     TACGAAGTGCGCAACGTGTCCGGGGTGTACTATGTCACG39     AACGACTGTTCCAACTCAAGCATTGTGTATGAGACAGCG78     GACATGATCATGCACACCCCTGGGTGCGTGCCCTGCGTT117     CGGGAGAGCAATTCCTCCCGCTGCTGGGTAGCGCTTACT156     CCCACGCTCGCGGCCAGGAACGCCAGCGTCCCCACTAAG195     ACAATACGACGTCACGTCGACTTGCTCGTTGGGGCGGCT234     GCTTTCTGTTCCGCTATGTACGTGGGGGATCTCTGCGGA273     TCTGTTTTCCTCGTCTCCCAGCTGTTCACTTTCTCGCCT312     CGCCGGCATGAGACAGTACAGGACTGCAACTGCTCAATC351     TATCCCGGCCACGTAACAGGTCACCGTATGGCTTGGGAT390     ATGATGATGAACTGGTCGCCCACAACGGCACTAGTGGTG429     TCGCAGTTGCTCCGGATCCCACAAGCTGTCGTGGACATG468     GTGGCGGGGGCCCACTGGGGAGTCCTGGCGGGCCTTGCC507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTTTTGATT546     GTGCTGCTACTCTTTGCCGGCGTTGATGGG576     (2) INFORMATION FOR SEQ ID NO:24:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24:     TATGAAGTGCGCAACGTGTCCGGGATGTACCATGTCACG39     AACGACTGCTCCAACTCAAGCATTGTGTTTGAGGCAGCG78     GACTTGATCATGCACACCCCCGGGTGCGTGCCCTGCGTT117     CGGGAGGGCAACTCCTCCCGCTGCTGGGTAGCGCTCACT156     CCCACGCTCGCGGCCAGGAACACCAGCGTCCCCACTACG195     ACGATACGACGCCATGTCGATTTGCTCGTTGGGGCGGCT234     GCTTTCTGCTCCGCTATGTATGTGGGAGACCTCTGCGGA273     TCTGTTTTCCTCGTCTCTCAGCTGTTCACCTTCTCGCCT312     CGCCGGCATGAGACTTTGCAGGACTGCAACTGCTCAATC351     TATCCCGGCCATCTGTCAGGTCACCGCATGGCTTGGGAC390     ATGATGATGAACTGGTCGCCTACAACAGCTCTAGTGGTG429     TCGCAGTTACTCCGGATCCCACAAGCTGTCATGGACATG468     GTGACAGGGGCCCACTGGGGAGTCCTGGCGGGCCTTGCC507     TACTATTCCATGGCGGGGAACTGGGCTAAGGTTTTAATT546     GTGATGCTACTCTTTGCCGGCGTTGATGGG576     (2) INFORMATION FOR SEQ ID NO:25:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: US6     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25:     TATGAAGTGCGCAACGTGTCCGGGATGTACCATGTCACG39     AACGACTGCTCCAACTCAAGCATTGTGTATGAGGCAGCG78     GACATGATCATGCACACTCCCGGGTGCGTGCCCTGTGTT117     CGGGAGAACAATTCCTCCCGCTGCTGGGTAGCGCTCACT156     CCCACGCTCGCGGCCAGGAACGCTAGCGTCCCCACTACG195     ACAATACGACGCCACGTCGATTTGCTCGTTGGGGCGGCT234     ACTTTCTGCTCCGCTATGTACGTGGGGGACCTCTGCGGG273     TCCGTTTTCCTCATCTCCCAGCTGTTCACCTTCTCGCCT312     CGTCAGCATGAGACAGTACAGGACTGCAATTGTTCAATC351     TATCCCGGCCACGTATCAGGTCACCGCATGGCTTGGGAT390     ATGATGATGAATTGGTCACCTACAGCAGCCCTAGTGGTA429     TCGCAGTTACTCCGGATCCCACAAGCTGTCATGGACATG468     GTGGCGGGGGCCCACTGGGGAGTCCTGGCGGGCCTTGCC507     TACTATTCCATGGTGGGGAACTGGGCTAAGGTTCTGATT546     GTGTTGCTACTCTTTGCCGGCGTTGACGGG576     (2) INFORMATION FOR SEQ ID NO:26:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T2     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26:     GCCCAAGTGAGGAACACCAGCCGCGGTTACATGGTGACT39     AACGACTGTTCCAATGAGAGCATCACCTGGCAGCTCCAA78     GCCGCGGTTCTCCACGTCCCCGGGTGTATCCCGTGTGAG117     AGGCTGGGAAATACATCCCGATGCTGGATACCGGTCACA156     CCAAACGTGGCCGTGCGGCAGCCCGGCGCTCTTACGCAG195     GGCTTGCGGACGCACATCGACATGGTTGTGATGTCCGCC234     ACGCTCTGCTCTGCCCTCTACGTGGGGGACCTCTGCGGC273     GGGGTGATGCTCGCAGCCCAGATGTTCATTGTCTCGCCG312     CGACGCCACTGGTTTGTGCAAGAATGCAATTGCTCCATC351     TACCCCGGTACCATCACTGGACACCGTATGGCATGGGAC390     ATGATGATGAACTGGTCGCCCACAGCCACCATGATCCTG429     GCGTACGCGATGCGCGTTCCCGAGGTCATCATAGACATC468     ATCGGCGGGGCTCACTGGGGCGTCATGTTTGGCTTGGCC507     TACTTCTCTATGCAGGGAGCGTGGGCGAAGGTCATTGTC546     ATCCTCTTGCTGGCTGCTGGGGTGGACGCG576     (2) INFORMATION FOR SEQ ID NO:27:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27:     GCACAAGTGAAGAACACCACTAACAGCTACATGGTGACC39     AACGACTGTTCTAATGACAGCATCACTTGGCAGCTCCAG78     GCCGCGGTCCTCCACGTCCCCGGGTGTGTCCCGTGCGAG117     AAAACGGGAAATACATCTCGGTGCTGGATACCGGTTTCA156     CCAAACGTGGCCGTGCGGCAGCCCGGCGCCCTCACGCAG195     GGCTTGCGGACGCACATTGACATGGTTGTGATGTCCGCC234     ACGCTCTGCTCTGCTCTTTACGTGGGGGACCTCTGCGGC273     GGGGTGATGCTCGCAGCCCAGATGTTCATCGTCTCGCCG312     CAACATCACTGGTTTGTGCAAGACTGCAATTGCTCTATC351     TACCCTGGCACCATCACTGGACACCGTATGGCATGGGAT390     ATGATGATGAACTGGTCGCCCACGGCCACCATGATCCTG429     GCGTACGCGATGCGCGTTCCCGAGGTCATCTTAGACATC468     GTTAGCGGGGCACACTGGGGCGTCATGTTCGGCTTGGCC507     TACTTCTCTATGCAGGGAGCGTGGGCGAAAGTCGTTGTC546     ATCCTTCTGCTGGCCGCTGGGGTGGACGCG576     (2) INFORMATION FOR SEQ ID NO:28:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T9     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28:     GCCGAAGTGAAGAACACCAGTACCAGCTACATGGTGACA39     AATGACTGTTCCAACGACAGCATCACCTGGCAACTCCAG78     GCCGCGGTCCTCCACGTCCCCGGGTGCGTCCCGTGCGAG117     AGAGTTGGAAACGCGTCGCGGTGCTGGATACCGGTCTCG156     CCAAACGTAGCTGTGCAGCGGCCTGGCGCCCTCACGCAG195     GGCTTGCGGACGCACATCGACATGGTTGTGATGTCCGCC234     ACGCTCTGCTCCGCTCTCTACGTGGGGGATCTCTGCGGC273     GGGGTAATGCTCGCCGCTCAGATGTTCATTATCTCGCCG312     CAGCACCACTGGTTTGTGCAGGAATGCAACTGCTCCATT351     TACCCTGGTACCATCACTGGACACCGTATGGCATGGGAC390     ATGATGATGAACTGGTCGCCCACAACCACCATGATCTTG429     GCGTACGCGATGCGCGTTCCCGAGGTCATCATAGACATC468     ATCAGCGGAGCTCACTGGGGCGTCATGTTCGGCCTAGCC507     TACTTCTCTATGCAGGGAGCGTGGGCGAAGGTCGTTGTC546     ATCCTGTTGCTCACCGCTGGCGTGGACGCG576     (2) INFORMATION FOR SEQ ID NO:29:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: 10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29:     GTCCAAGTGAAAAACACCAGTACCAGCTATATGGTGACC39     AATGACTGCTCCAACGACAGCATCACTTGGCAACTTGAG78     GCTGCGGTCCTCCACGTTCCCGGGTGTGTCCCGTGCGAG117     AAAGTGGGAAATACATCTCGGTGCTGGATACCGGTCTCA156     CCAAATGTGGCCGTGCAGCGGCCTGGCGCCCTCACGCAG195     GGCTTGCGGACTCACATCGACATGGTCGTGATGTCCGCC234     ACGCTCTGCTCCGCTCTTTACGTGGGGGACTTCTGCGGT273     GGGATGATGCTCGCAGCCCAAATGTTCATTGTCTCGCCG312     CGCCACCACTCGTTTGTGCAGGAATGCAACTGCTCCATC351     TACCCCGGTACCATCACCGGGCACCGTATGGCATGGGAC390     ATGATGATGAACTGGTCGCCCACGGCCACTTTGATCCTG429     GCGTACGTGATGCGCGTTCCCGAGGTCATCATAGACATC468     ATTAGCGGGGCGCATTGGGGCGTCTTGTTCGGCTTAGCC507     TACTTCTCTATGCAGGGAGCGTGGGCGAAAGTCGTTGTC546     ATCCTTCTGCTAGCCGCTGGGGTGGACGCG576     (2) INFORMATION FOR SEQ ID NO:30:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK8     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30:     GTGGAAGTCAGGAACATCAGTTCCAGCTACTACGCCACC39     AATGATTGCTCAAACAACAGCATCACCTGGCAACTCACC78     GACGCAGTTCTCCACCTTCCCGGATGCGTCCCATGTGAG117     AATGACAATGGCACCCTGCGCTGCTGGATACAAGTGACA156     CCTAATGTGGCTGTGAAACACCGCGGCGCACTTACTCAT195     AACCTGCGAACACACGTCGACGTGATCGTAATGGCAGCT234     ACGGTCTGCTCGGCCTTGTATGTGGGAGACGTATGCGGG273     GCCGTGATGATCGTGTCGCAGGCTCTCATAATATCGCCT312     GAACGCCACAACTTTACCCAGGAGTGCAACTGTTCCATC351     TACCAAGGTCATATCACCGGCCACCGCATGGCATGGGAC390     ATGATGCTAAACTGGTCACCAACTCTTACCATGATCCTC429     GCCTATGCCGCTCGTGTTCCTGAGCTAGCCCTCCAGGTT468     GTCTTCGGCGGCCATTGGGGCGTGGTGTTTGGCTTGGCC507     TATTTCTCCATGCAGGGAGCGTGGGCCAAAGTCATTGCC546     ATCCTCCTTCTTGTCGCAGGAGTGGATGCA576     (2) INFORMATION FOR SEQ ID NO:31:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK11     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31:     GTGGAAGTCAGGAACACCAGTTCTAGTTACTACGCCACC39     AATGATTGCTCAAACAACAGCATCACCTGGCAACTCACC78     AACGCAGTTCTCCACCTTCCCGGATGCGTCCCATGTGAG117     AATGACAATGGCACCCTGCACTGCTGGATACAAGTGACA156     CCTAATGTGGCTGTGAAACACCGCGGCGCACTCACTCAC195     AACCTGCGAGCACATATAGATATGATTGTAATGGCAGCT234     ACGGTCTGCTCGGCCTTGTATGTGGGAGACGTGTGCGGG273     GCCGTGATGATCGTGTCGCAGGCTTTCATAGTATCGCCA312     GAACACCACCACTTTACCCAAGAGTGCAACTGTTCCATC351     TACCAAGGTCACATCACCGGCCACCGCATGGCATGGGAC390     ATGATGCTTAACTGGTCACCAACTCTCACCATGATCCTC429     GCCTATGCCGCCCGTGTTCCTGAGCTAGTCCTTGAAGTC468     GTCTTCGGTGGTCATTGGGGTGTGGTGTTTGGCTTGGCC507     TATTTCTCCATGCAGGGAGCGTGGGCCAAGGTCATTGCC546     ATCCTCCTTCTTGTAGCAGGAGTGGATGCA576     (2) INFORMATION FOR SEQ ID NO:32:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SW3     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32:     GTGGAAGTCAGGAACATCAGTTCTAGCTACTATGCCACC39     AATGATTGCTCAAACAGCAGCATCACCTGGCAACTCACC78     AACGCAGTCCTCCACCTTCCCGGATGCGTCCCGTGTGAG117     AATGATAATGGCACCCTGCACTGCTGGATACAAGTGACA156     CCTAATGTGGCTGTGAAACACCGCGGCGCGCTCACTCAC195     AACCTGCGAGCACACGTCGATATGATCGTAATGGCAGCT234     ACGGTCTGCTCGGCCTTGTATGTGGGAGACATGTGCGGG273     GCCGTGATGATCGTGTCGCAGGCTTTCATAATATCGCCA312     GAACGCCACAACTTTACCCAAGAGTGCAACTGTTCCATC351     TACCAAGGTCGTATCACCGGCCACCGCATGGCGTGGGAC390     ATGATGCTAAACTGGTCACCAACTCTTACCATGATCCTT429     GCCTATGCCGCTCGTGTTCCTGAGCTAGTCCTTGAAGTT468     GTCTTCGGCGGCCATTGGGGCGTGGTGTTTGGCTTGGCC507     TATTTCTCCATGCAAGGAGCGTGGGCCAAGGTCATTGCC546     ATCCTCCTGCTTGTCGCAGGAGTGGATGCA576     (2) INFORMATION FOR SEQ ID NO:33:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T8     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33:     GTGGAAGTTAGAAACACCAGTTTTAGCTACTACGCCACC39     AATGATTGCTCGAACAACAGCATCACCTGGCAGCTCACC78     AACGCAGTTCTCCACCTTCCCGGATGCGTCCCATGTGAG117     AATGACAATGGCACCTTGCGCTGCTGGATACAAGTAACA156     CCTAATGTGGCTGTGAAACACCGTGGCGCACTCACTCAC195     AACCTGCGAACGCATGTCGACGTGATCGTAATGGCAGCT234     ACGGTCTGCTCGGCCTTGTATGTGGGGGACGTGTGCGGG273     GCCGTGATGATAGCGTCGCAGGCTTTCATAATATCGCCA312     GAACGCCACAACTTCACCCAGGAGTGCAACTGTTCCATC351     TACCAAGGTCATATCACCGGCCACCGCATGGCATGGGAC390     ATGATGCTGAACTGGTCACCAACTCTCACCATGATCCTC429     GCCTACGCTGCTCGTGTGCCTGAACTAGTCCTTGAAGTT468     GTCTTCGGCGGCCATTGGGGCGTGGTGTTTGGCTTGGCC507     TATTTCTCCATGCAAGGAGCGTGGGCCAAAGTCATCGCC546     ATCCTCCTCCTTGTCGCAGGAGTGGACGCA576     (2) INFORMATION FOR SEQ ID NO:34:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S83     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34:     GTGGAGGTCAAGGACACCGGCGACTCCTACATGCCGACC39     AACGATTGCTCCAACTCTAGTATCGTTTGGCAGCTTGAA78     GGAGCAGTGCTTCATACTCCTGGATGCGTCCCTTGTGAG117     CGTACCGCCAACGTCTCTCGATGTTGGGTGCCGGTTGCC156     CCCAATCTCGCCATAAGTCAACCTGGCGCTCTCACTAAG195     GGCCTGCGAGCACACATCGATATCATCGTGATGTCTGCT234     ACGGTCTGTTCTGCCCTTTATGTGGGGGACGTGTGTGGC273     GCGCTGATGCTGGCCGCTCAGGTCGTCGTCGTGTCGCCA312     CAACACCATACGTTTGTCCAGGAATGCAACTGTTCCATA351     TACCCGGGCCGCATTACGGGACACCGCATGGCTTGGGAT390     ATGATGATGAACTGGTCGCCCACTACCACCATGCTCCTG429     GCGTACTTGGTGCGCATCCCGGAAGTCATCTTGGATATT468     GTTACAGGAGGTCATTGGGGTGTAATGTTTGGCCTCGCT507     TACTTCTCCATGCAGGGATCGTGGGCGAAGGTCATCGTT546     ATCCTCCTGCTGACTGCTGGGGTGGAGGCG576     (2) INFORMATION FOR SEQ ID NO:35:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK12     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35:     TTAGAGTGGCGGAATGTGTCCGGCCTCTACGTCCTTACC39     AACGACTGTTCCAATAGCAGTATCGTGTATGAGGCCGAT78     GACGTCATTCTGCACACACCTGGCTGTGTACCTTGTGTT117     CAGGACGGCAATACATCTACGTGCTGGACCTCAGTGACG156     CCTACAGTGGCAGTCAGGTACGTCGGAGCAACCACCGCT195     TCGATACGCAGTCATGTGGACCTGCTAGTGGGCGCGGCC234     ACGATGTGCTCTGCGCTCTACGTGGGTGATGTGTGTGGG273     GCCGTCTTCCTTGTGGGACAAGCCTTCACGTTCAGACCT312     CGTCGCCATCAAACAGTCCAGACCTGTAACTGCTCGCTG351     TACCCAGGCCATCTTTCAGGACATCGAATGGCTTGGGAT390     ATGATGATGAATTGGTCCCCCGCTGTGGGTATGGTGGTA429     GCGCACGTCCTGCGTCTGCCCCAGACCTTGTTCGACATA468     ATAGCTGGGGCCCATTGGGGCATCATGGCGGGCCTAGCC507     TATTACTCCATGCAGGGCAACTGGGCCAAGGTCGCTATC546     ATCATGGTTATGTTTTCAGGAGTCGATGCC576     (2) INFORMATION FOR SEQ ID NO:36:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36:     CTAGAGTGGCGGAATGTGTCTGGCCTCTATGTCCTTACC39     AACGACTGTCCCAATAGCAGTATTGTGTATGAGGCCGAT78     GACGTCATTCTGCACACACCTGGCTGTGTACCTTGTGTT117     CAGGACGGCAATACATCCACGTGCTGGACCTCGGTGACA156     CCTACAGTGGCAGTCAGGTACGTCGGAGCAACCACCGCC195     TCGATACGCAGTCATGTGGACCTGTTAGTGGGCGCGGCC234     ACGATGTGCTCTGCGCTCTACGTGGGCGATATGTGTGGG273     GCCGTCTTCCTCGTGGGACAAGCCTTCACGTTCAGACCG312     CGTCGCCATCAAACGGTCCAGACCTGTAACTGCTCGCTG351     TACCCAGGCCACCTTTCAGGACATCGAATGGCTTGGGAT390     ATGATGATGAATTGGTCCCCCGCCGTGGGTATGGTGGTG429     GCGCACGTCCTGCGGTTGCCCCAGACCTTGTTCGACATA468     ATAGCCGGGGCCCATTGGGGCATCTTGGCAGGCCTAGCC507     TATTACTCCATGCAGGGCAACTGGGCCAAGGTCGCTATC546     ATCATGGTTATGTTTTCAGGGGTCGATGCC576     (2) INFORMATION FOR SEQ ID NO:37:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S2     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37:     CTAGAGTGGCGGAATACGTCTGGCCTCTATGTCCTCACC39     AACGACTGTTCCAATAGCAGTATTGTGTATGAGGCCGAT78     GACGTTATTCTGCACACACCTGGCTGTGTACCTTGTGTT117     CAGGACGGTAATACATCCACGTGCTGGACCCCAGTGACA156     CCTACAGTGGCAGTCAGGTATGTCGGAGCAACCACCGCT195     TCGATACGCAGTCATGTGGACCTATTGGTGGGCGCGGCC234     ACTATGTGCTCTGCGCTCTACGTGGGTGATATGTGTGGG273     GCCGTCTTTCTCGTGGGACAAGCCTTCACGTTCAGACCT312     CGTCGCCATCAAACGGTCCAGACCTGTAACTGCTCGCTG351     TACCCAGGCCATCTTTCAGGACATCGCATGGCTTGGGAT390     ATGATGATGAATTGGTCCCCCGCTGTGGGTATGGTGGTG429     GCGCACGTTCTGCGTTTGCCCCAGACCGTGTTCGACATA468     ATAGCCGGGGCCCATTGGGGCATCTTGGCGGGCCTAGCC507     TATTACTCCATGCAAGGCAACTGGGCCAAGGTCGCTATC546     ATCATGGTTATGTTTTCAGGGGTCGACGCC576     (2) INFORMATION FOR SEQ ID NO:38:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S52     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38:     CTAGAGTGGCGGAATACGTCTGGCCTCTATGTCCTTACC39     AACGACTGTTCCAATAGCAGTATTGTGTATGAGGCCGAT78     GACGTCATTCTGCACACACCCGGCTGTGTACCTTGTGTT117     CAGGACGGCAATACATCCATGTGCTGGACCCCAGTGACA156     CCTACGGTGGCAGTCAGGTACGTCGGAGCAACCACCGCT195     TCGATACGCAGTCATGTGGACCTATTAGTGGGCGCGGCC234     ACGCTGTGCTCTGCGCTCTATGTGGGTGATATGTGTGGG273     GCCGTCTTTCTCGTGGGACAAGCCTTCACGTTCAGACCT312     CGTCGCCATCAAACGGTCCAGACCTGTAACTGCTCGCTG351     TACCCAGGCCATGTTTCAGGACATCGAATGGCTTGGGAT390     ATGATGATGAATTGGTCCCCCGCTGTGGGTATGGTGGTG429     GCGCACATCCTGCGATTGCCCCAGACCTTGTTTGACATA468     CTGGCCGGGGCCCATTGGGGCATCTTGGCGGGCCTAGCC507     TATTATTCTATGCAGGGCAACTGGGCCAAGGTCGCTATT546     GTCATGATTATGTTTTCAGGGGTCGATGCC576     (2) INFORMATION FOR SEQ ID NO:39:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S54     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39:     CTAGAGTGGCGGAATACGTCTGGCCTCTATATCCTTACC39     AACGACTGTTCCAATAGCAGTATTGTGTATGAGGCCGAT78     GACGTCATTCTGCACACACCCGGCTGTGTACCTTGTGTT117     CAGGACGGCAATACATCCACGTGCTGGACCCCAGTGACA156     CCTACGGTGGCAGTCAGGTACGTCGGAGCAACCACCGCT195     TCGATACGCAGTCATGTGGACCTATTAGTGGGCGCGGCC234     ACGCTGTGCTCTGCGCTCTATGTGGGTGATATGTGTGGG273     GCCGTCTTTCTCGTGGGACAAGCCTTCACGTTCAGACCT312     CGTCGCCATCAAACGGTCCAGACCTGTAACTGCTCGCTG351     TACCCAGGCCATCTTTCAGGACATCGAATGGCTTGGGAT390     ATGATGATGAATTGGTCCCCCGCTGTGGGTATGGTGGTG429     GCGCACATCCTGCGATTGCCCCAGACCTTGTTTGACATA468     CTGGCCGGGGCCCATTGGGGCATCTTGGCGGGCCTAGCC507     TATTATTCTATGCAGGGCAACTGGGCCAAGGTCGCTATC546     ATCATGATTATGTTTTCAGGGGTCGATGCC576     (2) INFORMATION FOR SEQ ID NO:40:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: Z4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40:     GAGCACTACCGGAATGCTTCGGGCATCTATCACATCACC39     AATGATTGTCCGAATTCCAGTATAGTCTATGAAGCTGAC78     CATCACATCCTACACTTGCCGGGGTGCGTACCCTGTGTG117     ATGACTGGGAACACATCGCGTTGCTGGACGCCGGTGACG156     CCTACAGTGGCTGTCGCACACCCGGGCGCTCCGCTTGAG195     TCGTTCCGGCGACATGTGGACTTAATGGTAGGCGCGGCC234     ACTTTGTGTTCTGCCCTCTATGTTGGGGACCTCTGCGGA273     GGTGCCTTCCTGATGGGGCAGATGATCACTTTTCGGCCG312     CGTCGCCACTGGACCACGCAGGAGTGCAATTGTTCCATC351     TACACTGGCCATATCACCGGCCACAGGATGGCGTGGGAC390     ATGATGATGAACTGGAGCCCTACCACCACTCTGCTCCTC429     GCCCAGATCATGAGGGTCCCCACAGCCTTTCTCGACATG468     GTTGCCGGAGGCCACTGGGGCGTCCTCGCGGGCTTGGCG507     TACTTCAGCATGCAAGGCAATTGGGCCAAGGTAGTCCTG546     GTCCTTTTCCTCTTTGCTGGGGTAGACGCC576     (2) INFORMATION FOR SEQ ID NO:41:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: Z1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41:     GTGCACTACCGGAATGCTTCGGGCGTCTATCATGTCACC39     AATGATTGCCCTAACACCAGCATAGTGTACGAGACGGAG78     CACCACATCATGCACTTGCCAGGGTGTGTCCCCTGTGTG117     CGGACGGAGAATACTTCTCGCTGCTGGGTGCCCTTGACC156     CCCACTGTGGCCGCGCCCTATCCCAACGCACCGTTAGAG195     TCCATGCGCAGGCATGTAGACCTGATGGTGGGTGCGGCT234     ACTATGTGTTCCGCCTTCTACATTGGAGATCTGTGTGGA273     GGCGTCTTCCTAGTGGGCCAGCTGTTCGACTTCCGACCG312     CGCCGGCACTGGACCACCCAGGATTGCAACTGCTCCATC351     TATCCTGGTCACGTCTCGGGCCACAGGATGGCCTGGGAC390     ATGATGATGAACTGGAGCCCTACCAGCGCGCTGATTATG429     GCTCAGATCTTACGGATCCCCTCTATCCTAGGTGACTTG468     CTCACCGGGGGTCACTGGGGAGTTCTTGCTGGTCTAGCT507     TTCTTCAGCATGCAGAGTAACTGGGCGAAGGTCATCCTG546     GTCCTATTCCTCTTTGCCGGGGTCGAGGGA576     (2) INFORMATION FOR SEQ ID NO:42:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: Z6     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42:     GTTAACTATCGCAATGCCTCGGGCGTCTATCACGTCACC39     AACGACTGCCCGAACTCGAGCATAGTGTATGAGGCCGAA78     CACCAGATCTTACACCTCCCAGGGTGCTTGCCCTGTGTG117     AGGGTTGGGAATCAGTCACGCTGCTGGGTGGCCCTTACT156     CCCACCGTGGCGGTGTCTTATATCGGTGCTCCGCTTGAC195     TCCCTCCGGAGACATGTGGACCTGATGGTGGGCGCCGCT234     ACTGTATGCTCTGCCCTCTACGTTGGAGATCTGTGCGGT273     GGTGCATTCTTGGTTGGCCAGATGTTCTCCTTCCAGCCG312     CGACGCCACTGGACTACGCAGGACTGCAATTGTTCTATC351     TACGCAGGGCATATCACGGGCCACAGGATGGCATGGGAC390     ATGATGATGAACTGGAGTCCCACAACCACCCTGCTTCTC429     GCCCAGGTCATGAGGATCCCTAGCACTCTGGTAGATCTA468     CTCGCTGGAGGGCACTGGGGCGTCCTTGTTGGGTTGGCG507     TACTTCAGTATGCAAGCTAATTGGGCCAAAGTCATCCTG546     GTCCTTTTCCTCTTCGCTGGAGTTGATGCC576     (2) INFORMATION FOR SEQ ID NO:43:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: Z7     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43:     GTCAACTATCACAATGCCTCGGGCGTCTATCACATCACC39     AACGACTGCCCGAACTCGAGCATAATGTATGAGGCCGAA78     CACCACATCCTACACCTCCCAGGGTGCGTACCCTGTGTG117     AGGGAGGGGAACCAGTCACGCTGCTGGGTGGCCCTTACT156     CCCACCGTGGCGGCGCCTTATATCGGTGCACCGCTTGAA195     TCCATCCGGAGACATGTGGACCTGATGGTAGGCGCTGCT234     ACAGTGTGCTCCGCTCTCTACATTGGGGACCTGTGCGGT273     GGCGTATTTTTGGTTGGTCAGATGTTTTCTTTCCAGCCG312     CGACGCCACTGGACTACGCAGGACTGCAATTGTTCCATC351     TATGCGGGGCACGTTACAGGCCACAGAATGGCATGGGAC390     ATGATGATGAACTGGAGTCCCACAACCACCTTGGTCCTC429     GCCCAGGTTATGAGGATCCCTAGCACTCTGGTGGACCTA468     CTCACTGGAGGGCACTGGGGTATCCTTATCGGGGTGGCA507     TACTTCTGCATGCAAGCTAATTGGGCCAAGGTCATTCTG546     GTCCTTTTCCTCTACGCTGGAGTTGATGCC576     (2) INFORMATION FOR SEQ ID NO:44:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK13     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44:     TACAACTATCGCAACAGCTCGGGTGTCTACCATGTCACC39     AACGATTGCCCGAACTCGAGCATAGTCTATGAAACCGAT78     TACCACATCTTACACCTCCCGGGATGCGTTCCTTGCGTG117     AGGGAAGGGAACAAGTCTACATGCTGGGTGTCTCTCACC156     CCCACCGTGGCTGCGCAACATCTGAATGCTCCGCTTGAG195     TCTTTGAGACGTCACGTGGATCTGATGGTGGGCGGCGCC234     ACTCTCTGCTCCGCCCTCTACATCGGAGACGTGTGTGGG273     GGTGTGTTCTTGGTCGGTCAACTGTTCACCTTCCAACCT312     CGCCGCCACTGGACCACCCAAGACTGCAATTGTTCCATC351     TACACAGGACATATCACAGGACACAGAATGGCTTGGGAC390     ATGATGATGAATTGGAGCCCCACTGCGACGCTGGTCCTC429     GCCCAACTTATGAGGATCCCAGGCGCCATGGTCGACCTG468     CTTGCAGGCGGCCACTGGGGCATTCTGGTTGGCATAGCG507     TACTTCAGCATGCAAGCTAATTGGGCCAAGGTTATCCTG546     GTCCTGTTTCTCTTTGCTGGAGTCGACGCT576     (2) INFORMATION FOR SEQ ID NO:45:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:45:     GTTCCCTACCGGAATGCCTCTGGGGTTTACCATGTCACC39     AATGACTGCCCAAACTCCTCCATAGTCTACGAGGCTGAT78     AGCCTGATCTTGCACGCACCTGGCTGCGTGCCCTGTGTC117     AGGCAAGATAATGTCAGTAGGTGCTGGGTCCAAATCACC156     CCCACACTGTCAGCCCCGACCTTCGGAGCGGTCACGGCT195     CCTCTTCGGAGGGCCGTTGACTACTTAGCGGGAGGAGCT234     GCTCTCTGCTCCGCACTATACGTCGGCGACGCGTGCGGG273     GCAGTGTTTCTGGTAGGCCAAATGTTCACCTATAGGCCT312     CGCCAGCATACCACAGTGCAGGACTGCAACTGTTCCATT351     TACAGTGGCCATATCACCGGCCACCGGATGGCTTGGGAC390     ATGATGATGAATTGGTCACCTACGACAGCCTTGCTGATG429     GCCCAGATGCTACGGATCCCCCAGGTGGTCATAGACATC468     ATAGCCGGGGGCCACTGGGGGGTCTTGTTTGCCGCCGCA507     TACTTTGCGTCGGCCGCCAACTGGGCTAAGGTAGTGCTG546     GTTCTGTTCCTGTTTGCGGGGGTCGATGGC576     (2) INFORMATION FOR SEQ ID NO:46:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46:     GTTCCCTACCGAAACGCCTCTGGGGTTTATCATGTCACC39     AATGATTGCCCAAACTCTTCCATAGTTTACGAGGCTGAT78     AACCTGATCTTGCATGCACCTGGTTGCGTGCCTTGTGTC117     AGGCAAGATAATGTCAGTAAGTGCTGGGTCCAAATCACC156     CCCACGTTGTCAGCCCCGAATCTCGGAGCGGTCACGGCT195     CCTCTTCGGAGGGCCGTTGACTACTTAGCGGGAGGGGCT234     GCCCTCTGCTCCGCACTATACGTCGGGGACGCGTGCGGG273     GCAGTGTTTTTGGTAGGCCAAATGTTCACCTATAGGCCT312     CGCCAGCACACTACGGTGCAAGACTGCAATTGCTCTATT351     TACAGTGGCCATATCACCGGCCACCGGATGGCATGGGAC390     ATGATGATGAATTGGTCACCTACGACGGCCTTGCTGATG429     GCCCAGTTGCTACGGATTCCCCAGGTGGTCATCGACATC468     ATTGCCGGGGGCCACTGGGGGGTCTTGTTTGCCGCCGCA507     TATTTCGCGTCAGCGGCTAACTGGGCTAAGGTTATACTG546     GTCTTGTTTCTGTTTGCGGGGGTCGATGCC576     (2) INFORMATION FOR SEQ ID NO:47:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA5     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47:     GTCCCCTACCGAAATGCCTCTGGGGTTTATCATGTCACC39     AATGATTGCCCAAACTCTTCCATAGTCTACGAGGCTGAT78     AACCTGATTCTGCACGCACCTGGTTGCGTGCCCTGTGTC117     AAGGAAGGTAATGTCAGTAGGTGCTGGGTCCAAATCACC156     CCCACATTGTCAGCCCCGAACCTCGGAGCGGTCACGGCT195     CCTCTTCGGAGGGTCGTTGACTACTTAGCGGGAGGGGCT234     GCCCTCTGCTCCGCACTATACGTCGGGGACGCGTGCGGG273     GCAGTGTTCTTGGTAGGCCAAATGTTCACCTATAGGCCT312     CGCCAGCATACTACGGTGCAGGACTGCAACTGTTCCATT351     TACAGCGGCCATATCACCGGCCACCGAATGGCATGGGAC390     ATGATGATGAATTGGTCACCTACGACAGCCTTGGTGATG429     GCCCAGGTGCTACGGATTCCCCAAGTGGTCATTGACATC468     ATTGCCGGGGGCCACTGGGGGGTCTTGTTCGCCGTCGCA507     TACTTCGCGTCAGCGGCTAACTGGGCTAAGGTTGTGCTG546     GTCCTGTTTCTGTTTGCGGGGGTCGATGGC576     (2) INFORMATION FOR SEQ ID NO:48:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA6     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:48:     GTTCCTTACCGGAATGCCTCTGGGGTGTATCATGTTACC39     AATGATTGCCCAAACTCTTCCATAGTCTATGAGGCTGAT78     GACCTGATCCTACACGCACCTGGCTGCGTGCCCTGTGTC117     CGGAAGGATAATGTCAGTAGATGCTGGGTTCATATCACC156     CCCACACTATCAGCCCCGAGCCTCGGAGCGGTCACGGCT195     CCTCTTCGGAGGGCCGTTGATTACTTGGCGGGAGGGGCC234     GCCCTGTGCTCCGCGTTATACGTCGGAGACGTGTGCGGG273     GCATTGTTTTTGGTAGGCCAAATGTTCACCTATAGGCCT312     CGCCAGCATGCTACGGTACAGGACTGCAACTGCTCCATT351     TACAGTGGCCATATCACTGGCCACCGGATGGCATGGGAC390     ATGATGATGAATTGGTCACCCGCGACAGCCTTGGTGATG429     GCCCAAATGCTACGGATTCCCCAGGTGGTCATTGACATC468     ATTGCCGGGGGCCACTGGGGGGTCTTGTTCGCCGCTGCA507     TACTTCGCGTCGGCGGCTAACTGGGCTAAGGTTGTGCTG546     GTCTTGTTTCTGTTTGCGGGGGTTGATGCC576     (2) INFORMATION FOR SEQ ID NO:49:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA7     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:49:     GTCCCCTACCGAAATGCCTCCGGGGTTTATCATGTCACC39     AATGATTGCCCGAACTCTTCCATAGTCTATGAGGCTGAC78     AACCTGATCCTGCACGCACCTGGTTGCGTGCCCTGTGTC117     AGACAAAATAATGTCAGTAGGTGCTGGGTCCAAATCACC156     CCCACATTGTCAGCCCCGAACCTCGGAGCGGTCACGGCT195     CCTCTTCGGAGGGCCGTTGACTACCTAGCGGGAGGGGCT234     GCCCTCTGCTCCGCGCTATACGTCGGGGACGCGTGCGGG273     GCAGTGTTTTTGGTAGGCCAGATGTTCAGCTATAGGCCT312     CGCCAGCACACTACGGTGCAGGACTGCAACTGTTCCATT351     TACAGTGGCCATATCACCGGCCACCGAATGGCATGGGAC390     ATGATGATGAATTGGTCACCTACGACAGCCTTGGTGATG429     GCCCAGTTGCTACGGATTCCCCAGGTGGTCATCGACATC468     ATTGCCGGGGGCCACTGGGGGGTCTTGTTCGCCGCCGCA507     TATTTCGCGTCAGCGGCTAACTGGGCTAAGGTTGTGCTG546     GTCTTGTTTCTGTTTGCGGGGGTCGATGCC576     (2) INFORMATION FOR SEQ ID NO:50:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA13     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:50:     GTTCCCTACCGAAATGCCTCTGGGGTTTATCATGTCACC39     AATGATTGCCCAAACTCTTCCATCGTCTACGAGGCTGAT78     GACCTGATCTTACACGCACCTGGTTGCGTGCCCTGTGTT117     AGGCAGGGTAATGTCAGTAGGTGCTGGGTCCAGATCACC156     CCCACACTGTCAGCCCCGAGCCTCGGAGCGGTCACGGCT195     CCTCTTCGGAGGGCCGTTGACTACTTAGCGGGGGGGGCT234     GCCCTTTGCTCCGCGTTATACGTCGGAGACGCGTGCGGG273     GCAGTGTTTTTGGTAGGTCAAATGTTCACCTATAGCCCT312     CGCCGGCATAATGTTGTGCAGGACTGCAACTGTTCCATT351     TACAGTGGCCACATCACCGGCCACCGGATGGCATGGGAC390     ATGATGATGAATTGGTCACCTACAACAGCTTTGGTGATG429     GCCCAGTTGTTACGGATTCCCCAGGTGGTCATTGACATC468     ATTGCCGGGGCCCACTGGGGGGTCTTGTTCGCCGCCGCA507     TACTACGCGTCGGCGGCTAACTGGGCCAAGGTTGTGCTG546     GTCCTGTTTCTGTTTGCGGGGGTCGATGCC576     (2) INFORMATION FOR SEQ ID NO:51:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 576 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK2     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:51:     CTTACCTACGGCAACTCCAGTGGGCTATACCATCTCACA39     AATGATTGCCCCAACTCCAGCATCGTGCTGGAGGCGGAT78     GCTATGATCTTGCATTTGCCTGGATGCTTGCCTTGTGTG117     AGGGTCGATGATCGGTCCACCTGTTGGCATGCTGTGACC156     CCCACCCTGGCCATACCAAATGCTTCCACGCCCGCAACG195     GGATTCCGCAGGCATGTGGATCTTCTTGCGGGCGCCGCA234     GTGGTTTGCTCATCCCTGTACATCGGGGACCTGTGTGGC273     TCTCTCTTTTTGGCGGGACAACTATTCACCTTTCAGCCC312     CGCCGTCATTGGACTGTGCAAGACTGCAACTGCTCCATC351     TATACAGGCCACGTCACCGGCCACAGGATGGCTTGGGAC390     ATGATGATGAACTGGTCACCCACAACCACTCTGGTCCTA429     TCTAGCATCTTGAGGGTACCTGAGATTTGTGCGAGTGTG468     ATATTTGGTGGCCATTGGGGGATACTACTAGCCGTTGCC507     TACTTTGGCATGGCTGGCAACTGGCTAAAAGTTCTGGCT546     GTTCTGTTCCTATTTGCAGGGGTTGAAGCA576     (2) INFORMATION FOR SEQ ID NO:52:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK7     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:52:     TyrGlnValArgAsnSerThrGlyLeuTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAlaAspAlaIleLeu     202530     HisThrProGlyCysValProCysValArgGluGlyAsnValSer     354045     ArgCysTrpValAlaMetThrProThrValAlaThrArgAspGly     505560     LysLeuProThrAlaGlnLeuArgArgHisIleAspLeuLeuVal     657075     GlySerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlySerValPheLeuValGlyGlnLeuPheThrPheSerProArg     95100105     ArgHisTrpThrThrGlnGlyCysAsnCysSerIleTyrProGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValAlaGlnLeuLeuArgIlePro     140145150     GlnAlaIleLeuAspMetIleAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyIleAlaTyrPheSerMetValGlyAsnTrpAlaLysVal     170175180     LeuValValLeuLeuLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:53:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK9     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:53:     TyrGlnValArgAsnSerSerGlyLeuTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAlaAspAlaIleLeu     202530     HisSerProGlyCysValProCysValArgGluGlyAsnAlaSer     354045     LysCysTrpValAlaValAlaProThrValAlaThrArgAspGly     505560     LysLeuProAlaThrGlnLeuArgArgHisIleAspLeuLeuVal     657075     GlySerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlySerValPheLeuValGlyGlnLeuPheThrPheSerProArg     95100105     ArgHisTrpThrThrGlnAspCysAsnCysSerIleTyrProGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValMetAlaGlnLeuLeuArgIlePro     140145150     GlnAlaIleLeuAspMetIleAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyIleAlaTyrPheSerMetValGlyAsnTrpAlaLysVal     170175180     ValValValLeuLeuLeuPheThrGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:54:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DR1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:54:     HisGlnValArgAsnSerThrGlyLeuTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAlaAspAlaIleLeu     202530     HisAlaProGlyCysValProCysValArgGluGlyAsnAlaSer     354045     ArgCysTrpValAlaValThrProThrValAlaThrArgAspGly     505560     LysLeuProThrThrGlnLeuArgArgHisIleAspLeuLeuVal     657075     GlySerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlySerValPheLeuValGlyGlnLeuPheThrPheSerProArg     95100105     ArgHisTrpThrThrGlnAspCysAsnCysSerIleTyrProGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValMetAlaGlnLeuLeuArgIlePro     140145150     GlnAlaIleLeuAspMetIleAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyIleAlaTyrPheSerMetValGlyAsnTrpAlaLysVal     170175180     ValValValLeuLeuLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:55:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DR4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:55:     HisGlnValArgAsnSerThrGlyLeuTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAlaAspAlaIleLeu     202530     HisThrProGlyCysValProCysValArgGluGlyAsnThrSer     354045     ArgCysTrpValAlaValThrProThrValAlaThrArgAspGly     505560     LysLeuProThrThrGlnLeuArgArgHisIleAspLeuLeuVal     657075     GlySerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlySerValPheLeuValGlyGlnLeuPheThrPheSerProArg     95100105     HisHisTrpThrThrGlnAspCysAsnCysSerIleTyrProGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValAlaGlnLeuLeuArgIlePro     140145150     GlnAlaIleLeuAspMetIleAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyIleAlaTyrPheSerMetValGlyAsnTrpAlaLysVal     170175180     LeuValValLeuLeuLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:56:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S14     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:56:     TyrGlnValArgAsnSerThrGlyLeuTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluThrAlaAspAlaIleLeu     202530     HisAlaProGlyCysValProCysValArgGluGlyAsnThrSer     354045     ArgCysTrpValAlaMetThrProThrValAlaThrArgAspGly     505560     LysLeuProAlaThrGlnLeuArgArgTyrIleAspLeuLeuVal     657075     GlySerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlySerValPheLeuValGlyGlnLeuPheThrPheSerProArg     95100105     ArgLeuTrpThrThrGlnAspCysAsnCysSerIleTyrProGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValAlaGlnLeuLeuArgIlePro     140145150     GlnAlaIleLeuAspMetIleAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyIleAlaTyrPheSerMetValGlyAsnTrpAlaLysVal     170175180     LeuValValLeuLeuLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:57:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S18     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:57:     TyrGlnValArgAsnSerThrGlyLeuTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluThrAlaAspThrIleLeu     202530     HisSerProGlyCysValProCysValArgGluGlyAsnAlaSer     354045     ArgCysTrpValProValAlaProThrValAlaThrArgAspGly     505560     LysLeuProAlaThrGlnLeuArgArgHisIleAspLeuLeuVal     657075     GlySerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrIleSerProArg     95100105     ArgHisTrpThrThrGlnAspCysAsnCysSerIleTyrProGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValIleAlaGlnLeuLeuArgValPro     140145150     GlnAlaValLeuAspMetIleAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyIleAlaTyrPheSerMetAlaGlyAsnTrpAlaLysVal     170175180     LeuLeuValLeuLeuLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:58:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SW1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:58:     TyrGlnValArgAsnSerSerGlyLeuTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluThrAlaAspAlaIleLeu     202530     HisSerProGlyCysValProCysValArgGluAspGlyAlaPro     354045     LysCysTrpValAlaValAlaProThrValAlaThrArgAspGly     505560     LysLeuProAlaThrGlnLeuArgArgHisIleAspLeuLeuVal     657075     GlySerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisTrpThrThrGlnAspCysAsnCysSerIleTyrProGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValAlaGlnLeuLeuArgIlePro     140145150     GlnAlaValLeuAspMetIleAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyIleAlaTyrPheSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValLeuLeuLeuPheSerGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:59:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: US11     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:59:     TyrGlnValArgAsnSerThrGlyLeuTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAlaAspAlaIleLeu     202530     HisThrProGlyCysValProCysValArgGluGlyAsnAlaSer     354045     ArgCysTrpValAlaMetThrProThrValAlaThrArgAspGly     505560     LysLeuProThrThrGlnLeuArgArgHisIleAspLeuLeuVal     657075     GlySerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlySerValPheLeuValGlyGlnLeuPheThrPheSerProArg     95100105     ArgHisTrpThrThrGlnGlyCysAsnCysSerIleTyrProGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValAlaGlnLeuLeuArgIlePro     140145150     GlnAlaIleLeuAspMetIleAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyIleAlaTyrPheSerMetValGlyAsnTrpAlaLysVal     170175180     LeuValValLeuLeuLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:60:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: D1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:60:     TyrGluValArgAsnValSerGlyValTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluThrAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAspAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnGly     505560     AsnValProThrThrAlaIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuIleSerGlnLeuPheThrLeuSerProArg     95100105     ArgHisGluThrValGlnGluCysAsnCysSerIleTyrProGly     110115120     HisValThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValMetAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:61:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: D3     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:61:     TyrGluValArgAsnValSerGlyValTyrGlnValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluThrAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAspAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnSer     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnGluCysAsnCysSerIleTyrProGly     110115120     HisValThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:62:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:62:     TyrGluValArgAsnValSerGlyValTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaValAspValIleMet     202530     HisThrProGlyCysValProCysValArgGluAsnAsnHisSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnAla     505560     SerIleProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrAlaGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValLeuSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetAlaGlyAsnTrpAlaLysVal     170175180     LeuIleValLeuLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:63:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK3     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:63:     TyrGluValArgAsnValSerGlyIleTyrHisValThrAsnAsp     51015     CysSerAsnSerSerValValTyrGluThrAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAsnAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnVal     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerLeuTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:64:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:64:     HisGluValHisAsnValSerGlyIleTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAsnAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnAla     505560     SerIleProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgLeuPro     140145150     GlnAlaValMetAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:65:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK5     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:65:     TyrGluValArgAsnValSerGlyValTyrHisValThrAsnAsp     51015     CysSerAsnLeuSerIleValTyrGluThrThrAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAsnAsnSerSer     354045     ArgCysTrpValAlaLeuAlaProThrLeuAlaAlaArgAsnAla     505560     SerValProThrThrAlaIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:66:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK8     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:66:     TyrGluValArgAsnValSerGlyIleTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluThrAlaAspMetIleMet     202530     HisThrProGlyCysMetProCysValArgGluAsnAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnVal     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaIleValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:67:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: IND5     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:67:     TyrGluValArgAsnValSerGlyValTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluGlyAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnAla     505560     SerValSerThrThrThrIleArgHisHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyIleLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:68:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: IND8     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:68:     TyrGluValArgAsnValSerGlyValTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluGlyAsnPheSer     354045     SerCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnAla     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyIleLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:69:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: P10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:69:     TyrGluValArgAsnValSerGlyValTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAsnAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnSer     505560     SerValProThrThrAlaIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValLeuLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisTrpThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaIleLeuAspValValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:70:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S9     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:70:     TyrGluValArgAsnValSerGlyAlaTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAlaAspValIleMet     202530     HisThrProGlyCysValProCysValGlnGluGlyAsnSerSer     354045     GlnCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnAla     505560     ThrValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaValPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuIleSerGlnLeuPheThrIleSerProArg     95100105     ArgHisGluThrValGlnAsnCysAsnCysSerIleTyrProGly     110115120     HisValThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValMetAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:71:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S45     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:71:     TyrGluValArgAsnValSerGlyAlaTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaValAspValIleLeu     202530     HisThrProGlyCysValProCysValArgGluAsnAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnSer     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:72:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:72:     TyrGluValArgAsnValSerGlyMetTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAsnAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnSer     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgTyrGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     ArgValThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaIleValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:73:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SW2     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:73:     TyrGluValArgAsnValSerGlyValTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluThrAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAlaAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnThr     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerValMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:74:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T3     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:74:     TyrGluValArgAsnValSerGlyValTyrTyrValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluThrAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluSerAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnAla     505560     SerValProThrLysThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValValAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValLeuLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:75:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:75:     TyrGluValArgAsnValSerGlyMetTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValPheGluAlaAlaAspLeuIleMet     202530     HisThrProGlyCysValProCysValArgGluGlyAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnThr     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaAlaPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuValSerGlnLeuPheThrPheSerProArg     95100105     ArgHisGluThrLeuGlnAspCysAsnCysSerIleTyrProGly     110115120     HisLeuSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValMetAspMetValThrGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetAlaGlyAsnTrpAlaLysVal     170175180     LeuIleValMetLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:76:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: US6     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:76:     TyrGluValArgAsnValSerGlyMetTyrHisValThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAlaAspMetIleMet     202530     HisThrProGlyCysValProCysValArgGluAsnAsnSerSer     354045     ArgCysTrpValAlaLeuThrProThrLeuAlaAlaArgAsnAla     505560     SerValProThrThrThrIleArgArgHisValAspLeuLeuVal     657075     GlyAlaAlaThrPheCysSerAlaMetTyrValGlyAspLeuCys     808590     GlySerValPheLeuIleSerGlnLeuPheThrPheSerProArg     95100105     GlnHisGluThrValGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaAlaLeuValValSerGlnLeuLeuArgIlePro     140145150     GlnAlaValMetAspMetValAlaGlyAlaHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysVal     170175180     LeuIleValLeuLeuLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:77:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T2     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:77:     AlaGlnValArgAsnThrSerArgGlyTyrMetValThrAsnAsp     51015     CysSerAsnGluSerIleThrTrpGlnLeuGlnAlaAlaValLeu     202530     HisValProGlyCysIleProCysGluArgLeuGlyAsnThrSer     354045     ArgCysTrpIleProValThrProAsnValAlaValArgGlnPro     505560     GlyAlaLeuThrGlnGlyLeuArgThrHisIleAspMetValVal     657075     MetSerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlyGlyValMetLeuAlaAlaGlnMetPheIleValSerProArg     95100105     ArgHisTrpPheValGlnGluCysAsnCysSerIleTyrProGly     110115120     ThrIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaThrMetIleLeuAlaTyrAlaMetArgValPro     140145150     GluValIleIleAspIleIleGlyGlyAlaHisTrpGlyValMet     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlyAlaTrpAlaLysVal     170175180     IleValIleLeuLeuLeuAlaAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:78:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:78:     AlaGlnValLysAsnThrThrAsnSerTyrMetValThrAsnAsp     51015     CysSerAsnAspSerIleThrTrpGlnLeuGlnAlaAlaValLeu     202530     HisValProGlyCysValProCysGluLysThrGlyAsnThrSer     354045     ArgCysTrpIleProValSerProAsnValAlaValArgGlnPro     505560     GlyAlaLeuThrGlnGlyLeuArgThrHisIleAspMetValVal     657075     MetSerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlyGlyValMetLeuAlaAlaGlnMetPheIleValSerProGln     95100105     HisHisTrpPheValGlnAspCysAsnCysSerIleTyrProGly     110115120     ThrIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaThrMetIleLeuAlaTyrAlaMetArgValPro     140145150     GluValIleLeuAspIleValSerGlyAlaHisTrpGlyValMet     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlyAlaTrpAlaLysVal     170175180     ValValIleLeuLeuLeuAlaAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:79:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T9     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:79:     AlaGluValLysAsnThrSerThrSerTyrMetValThrAsnAsp     51015     CysSerAsnAspSerIleThrTrpGlnLeuGlnAlaAlaValLeu     202530     HisValProGlyCysValProCysGluArgValGlyAsnAlaSer     354045     ArgCysTrpIleProValSerProAsnValAlaValGlnArgPro     505560     GlyAlaLeuThrGlnGlyLeuArgThrHisIleAspMetValVal     657075     MetSerAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlyGlyValMetLeuAlaAlaGlnMetPheIleIleSerProGln     95100105     HisHisTrpPheValGlnGluCysAsnCysSerIleTyrProGly     110115120     ThrIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrThrMetIleLeuAlaTyrAlaMetArgValPro     140145150     GluValIleIleAspIleIleSerGlyAlaHisTrpGlyValMet     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlyAlaTrpAlaLysVal     170175180     ValValIleLeuLeuLeuThrAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:80:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: US10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:80:     ValGlnValLysAsnThrSerThrSerTyrMetValThrAsnAsp     51015     CysSerAsnAspSerIleThrTrpGlnLeuGluAlaAlaValLeu     202530     HisValProGlyCysValProCysGluLysValGlyAsnThrSer     354045     ArgCysTrpIleProValSerProAsnValAlaValGlnArgPro     505560     GlyAlaLeuThrGlnGlyLeuArgThrHisIleAspMetValVal     657075     MetSerAlaThrLeuCysSerAlaLeuTyrValGlyAspPheCys     808590     GlyGlyMetMetLeuAlaAlaGlnMetPheIleValSerProArg     95100105     HisHisSerPheValGlnGluCysAsnCysSerIleTyrProGly     110115120     ThrIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaThrLeuIleLeuAlaTyrValMetArgValPro     140145150     GluValIleIleAspIleIleSerGlyAlaHisTrpGlyValLeu     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlyAlaTrpAlaLysVal     170175180     ValValIleLeuLeuLeuAlaAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:81:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK8     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:81:     ValGluValArgAsnIleSerSerSerTyrTyrAlaThrAsnAsp     51015     CysSerAsnAsnSerIleThrTrpGlnLeuThrAspAlaValLeu     202530     HisLeuProGlyCysValProCysGluAsnAspAsnGlyThrLeu     354045     ArgCysTrpIleGlnValThrProAsnValAlaValLysHisArg     505560     GlyAlaLeuThrHisAsnLeuArgThrHisValAspValIleVal     657075     MetAlaAlaThrValCysSerAlaLeuTyrValGlyAspValCys     808590     GlyAlaValMetIleValSerGlnAlaLeuIleIleSerProGlu     95100105     ArgHisAsnPheThrGlnGluCysAsnCysSerIleTyrGlnGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetLeuAsnTrp     125130135     SerProThrLeuThrMetIleLeuAlaTyrAlaAlaArgValPro     140145150     GluLeuAlaLeuGlnValValPheGlyGlyHisTrpGlyValVal     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlyAlaTrpAlaLysVal     170175180     IleAlaIleLeuLeuLeuValAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:82:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK11     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:82:     ValGluValArgAsnThrSerSerSerTyrTyrAlaThrAsnAsp     51015     CysSerAsnAsnSerIleThrTrpGlnLeuThrAsnAlaValLeu     202530     HisLeuProGlyCysValProCysGluAsnAspAsnGlyThrLeu     354045     HisCysTrpIleGlnValThrProAsnValAlaValLysHisArg     505560     GlyAlaLeuThrHisAsnLeuArgAlaHisIleAspMetIleVal     657075     MetAlaAlaThrValCysSerAlaLeuTyrValGlyAspValCys     808590     GlyAlaValMetIleValSerGlnAlaPheIleValSerProGlu     95100105     HisHisHisPheThrGlnGluCysAsnCysSerIleTyrGlnGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetLeuAsnTrp     125130135     SerProThrLeuThrMetIleLeuAlaTyrAlaAlaArgValPro     140145150     GluLeuValLeuGluValValPheGlyGlyHisTrpGlyValVal     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlyAlaTrpAlaLysVal     170175180     IleAlaIleLeuLeuLeuValAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:83:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SW3     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:83:     ValGluValArgAsnIleSerSerSerTyrTyrAlaThrAsnAsp     51015     CysSerAsnSerSerIleThrTrpGlnLeuThrAsnAlaValLeu     202530     HisLeuProGlyCysValProCysGluAsnAspAsnGlyThrLeu     354045     HisCysTrpIleGlnValThrProAsnValAlaValLysHisArg     505560     GlyAlaLeuThrHisAsnLeuArgAlaHisValAspMetIleVal     657075     MetAlaAlaThrValCysSerAlaLeuTyrValGlyAspMetCys     808590     GlyAlaValMetIleValSerGlnAlaPheIleIleSerProGlu     95100105     ArgHisAsnPheThrGlnGluCysAsnCysSerIleTyrGlnGly     110115120     ArgIleThrGlyHisArgMetAlaTrpAspMetMetLeuAsnTrp     125130135     SerProThrLeuThrMetIleLeuAlaTyrAlaAlaArgValPro     140145150     GluLeuValLeuGluValValPheGlyGlyHisTrpGlyValVal     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlyAlaTrpAlaLysVal     170175180     IleAlaIleLeuLeuLeuValAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:84:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: T8     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:84:     ValGluValArgAsnThrSerPheSerTyrTyrAlaThrAsnAsp     51015     CysSerAsnAsnSerIleThrTrpGlnLeuThrAsnAlaValLeu     202530     HisLeuProGlyCysValProCysGluAsnAspAsnGlyThrLeu     354045     ArgCysTrpIleGlnValThrProAsnValAlaValLysHisArg     505560     GlyAlaLeuThrHisAsnLeuArgThrHisValAspValIleVal     657075     MetAlaAlaThrValCysSerAlaLeuTyrValGlyAspValCys     808590     GlyAlaValMetIleAlaSerGlnAlaPheIleIleSerProGlu     95100105     ArgHisAsnPheThrGlnGluCysAsnCysSerIleTyrGlnGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetLeuAsnTrp     125130135     SerProThrLeuThrMetIleLeuAlaTyrAlaAlaArgValPro     140145150     GluLeuValLeuGluValValPheGlyGlyHisTrpGlyValVal     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlyAlaTrpAlaLysVal     170175180     IleAlaIleLeuLeuLeuValAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:85:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S83     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:85:     ValGluValLysAspThrGlyAspSerTyrMetProThrAsnAsp     51015     CysSerAsnSerSerIleValTrpGlnLeuGluGlyAlaValLeu     202530     HisThrProGlyCysValProCysGluArgThrAlaAsnValSer     354045     ArgCysTrpValProValAlaProAsnLeuAlaIleSerGlnPro     505560     GlyAlaLeuThrLysGlyLeuArgAlaHisIleAspIleIleVal     657075     MetSerAlaThrValCysSerAlaLeuTyrValGlyAspValCys     808590     GlyAlaLeuMetLeuAlaAlaGlnValValValValSerProGln     95100105     HisHisThrPheValGlnGluCysAsnCysSerIleTyrProGly     110115120     ArgIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrThrMetLeuLeuAlaTyrLeuValArgIlePro     140145150     GluValIleLeuAspIleValThrGlyGlyHisTrpGlyValMet     155160165     PheGlyLeuAlaTyrPheSerMetGlnGlySerTrpAlaLysVal     170175180     IleValIleLeuLeuLeuThrAlaGlyValGluAla     185190     (2) INFORMATION FOR SEQ ID NO:86:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK12     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:86:     LeuGluTrpArgAsnValSerGlyLeuTyrValLeuThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAspAspValIleLeu     202530     HisThrProGlyCysValProCysValGlnAspGlyAsnThrSer     354045     ThrCysTrpThrSerValThrProThrValAlaValArgTyrVal     505560     GlyAlaThrThrAlaSerIleArgSerHisValAspLeuLeuVal     657075     GlyAlaAlaThrMetCysSerAlaLeuTyrValGlyAspValCys     808590     GlyAlaValPheLeuValGlyGlnAlaPheThrPheArgProArg     95100105     ArgHisGlnThrValGlnThrCysAsnCysSerLeuTyrProGly     110115120     HisLeuSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProAlaValGlyMetValValAlaHisValLeuArgLeuPro     140145150     GlnThrLeuPheAspIleIleAlaGlyAlaHisTrpGlyIleMet     155160165     AlaGlyLeuAlaTyrTyrSerMetGlnGlyAsnTrpAlaLysVal     170175180     AlaIleIleMetValMetPheSerGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:87:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK10     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:87:     LeuGluTrpArgAsnValSerGlyLeuTyrValLeuThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAspAspValIleLeu     202530     HisThrProGlyCysValProCysValGlnAspGlyAsnThrSer     354045     ThrCysTrpThrSerValThrProThrValAlaValArgTyrVal     505560     GlyAlaThrThrAlaSerIleArgSerHisValAspLeuLeuVal     657075     GlyAlaAlaThrMetCysSerAlaLeuTyrValGlyAspMetCys     808590     GlyAlaValPheLeuValGlyGlnAlaPheThrPheArgProArg     95100105     ArgHisGlnThrValGlnThrCysAsnCysSerLeuTyrProGly     110115120     HisLeuSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProAlaValGlyMetValValAlaHisValLeuArgLeuPro     140145150     GlnThrLeuPheAspIleIleAlaGlyAlaHisTrpGlyIleLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetGlnGlyAsnTrpAlaLysVal     170175180     AlaIleIleMetValMetPheSerGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:88:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S2     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:88:     LeuGluTrpArgAsnThrSerGlyLeuTyrValLeuThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAspAspValIleLeu     202530     HisThrProGlyCysValProCysValGlnAspGlyAsnThrSer     354045     ThrCysTrpThrProValThrProThrValAlaValArgTyrVal     505560     GlyAlaThrThrAlaSerIleArgSerHisValAspLeuLeuVal     657075     GlyAlaAlaThrMetCysSerAlaLeuTyrValGlyAspMetCys     808590     GlyAlaValPheLeuValGlyGlnAlaPheThrPheArgProArg     95100105     ArgHisGlnThrValGlnThrCysAsnCysSerLeuTyrProGly     110115120     HisLeuSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProAlaValGlyMetValValAlaHisValLeuArgLeuPro     140145150     GlnThrValPheAspIleIleAlaGlyAlaHisTrpGlyIleLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetGlnGlyAsnTrpAlaLysVal     170175180     AlaIleIleMetValMetPheSerGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:89:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S52     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:89:     LeuGluTrpArgAsnThrSerGlyLeuTyrValLeuThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAspAspValIleLeu     202530     HisThrProGlyCysValProCysValGlnAspGlyAsnThrSer     354045     MetCysTrpThrProValThrProThrValAlaValArgTyrVal     505560     GlyAlaThrThrAlaSerIleArgSerHisValAspLeuLeuVal     657075     GlyAlaAlaThrLeuCysSerAlaLeuTyrValGlyAspMetCys     808590     GlyAlaValPheLeuValGlyGlnAlaPheThrPheArgProArg     95100105     ArgHisGlnThrValGlnThrCysAsnCysSerLeuTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProAlaValGlyMetValValAlaHisIleLeuArgLeuPro     140145150     GlnThrLeuPheAspIleLeuAlaGlyAlaHisTrpGlyIleLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetGlnGlyAsnTrpAlaLysVal     170175180     AlaIleValMetIleMetPheSerGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:90:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: S54     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:90:     LeuGluTrpArgAsnThrSerGlyLeuTyrIleLeuThrAsnAsp     51015     CysSerAsnSerSerIleValTyrGluAlaAspAspValIleLeu     202530     HisThrProGlyCysValProCysValGlnAspGlyAsnThrSer     354045     ThrCysTrpThrProValThrProThrValAlaValArgTyrVal     505560     GlyAlaThrThrAlaSerIleArgSerHisValAspLeuLeuVal     657075     GlyAlaAlaThrLeuCysSerAlaLeuTyrValGlyAspMetCys     808590     GlyAlaValPheLeuValGlyGlnAlaPheThrPheArgProArg     95100105     ArgHisGlnThrValGlnThrCysAsnCysSerLeuTyrProGly     110115120     HisLeuSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProAlaValGlyMetValValAlaHisIleLeuArgLeuPro     140145150     GlnThrLeuPheAspIleLeuAlaGlyAlaHisTrpGlyIleLeu     155160165     AlaGlyLeuAlaTyrTyrSerMetGlnGlyAsnTrpAlaLysVal     170175180     AlaIleIleMetIleMetPheSerGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:91:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: Z4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:91:     GluHisTyrArgAsnAlaSerGlyIleTyrHisIleThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAspHisHisIleLeu     202530     HisLeuProGlyCysValProCysValMetThrGlyAsnThrSer     354045     ArgCysTrpThrProValThrProThrValAlaValAlaHisPro     505560     GlyAlaProLeuGluSerPheArgArgHisValAspLeuMetVal     657075     GlyAlaAlaThrLeuCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlyGlyAlaPheLeuMetGlyGlnMetIleThrPheArgProArg     95100105     ArgHisTrpThrThrGlnGluCysAsnCysSerIleTyrThrGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrThrLeuLeuLeuAlaGlnIleMetArgValPro     140145150     ThrAlaPheLeuAspMetValAlaGlyGlyHisTrpGlyValLeu     155160165     AlaGlyLeuAlaTyrPheSerMetGlnGlyAsnTrpAlaLysVal     170175180     ValLeuValLeuPheLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:92:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: Z1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:92:     ValHisTyrArgAsnAlaSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnThrSerIleValTyrGluThrGluHisHisIleMet     202530     HisLeuProGlyCysValProCysValArgThrGluAsnThrSer     354045     ArgCysTrpValProLeuThrProThrValAlaAlaProTyrPro     505560     AsnAlaProLeuGluSerMetArgArgHisValAspLeuMetVal     657075     GlyAlaAlaThrMetCysSerAlaPheTyrIleGlyAspLeuCys     808590     GlyGlyValPheLeuValGlyGlnLeuPheAspPheArgProArg     95100105     ArgHisTrpThrThrGlnAspCysAsnCysSerIleTyrProGly     110115120     HisValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrSerAlaLeuIleMetAlaGlnIleLeuArgIlePro     140145150     SerIleLeuGlyAspLeuLeuThrGlyGlyHisTrpGlyValLeu     155160165     AlaGlyLeuAlaPhePheSerMetGlnSerAsnTrpAlaLysVal     170175180     IleLeuValLeuPheLeuPheAlaGlyValGluGly     185190     (2) INFORMATION FOR SEQ ID NO:93:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: Z6     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:93:     ValAsnTyrArgAsnAlaSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaGluHisGlnIleLeu     202530     HisLeuProGlyCysLeuProCysValArgValGlyAsnGlnSer     354045     ArgCysTrpValAlaLeuThrProThrValAlaValSerTyrIle     505560     GlyAlaProLeuAspSerLeuArgArgHisValAspLeuMetVal     657075     GlyAlaAlaThrValCysSerAlaLeuTyrValGlyAspLeuCys     808590     GlyGlyAlaPheLeuValGlyGlnMetPheSerPheGlnProArg     95100105     ArgHisTrpThrThrGlnAspCysAsnCysSerIleTyrAlaGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrThrLeuLeuLeuAlaGlnValMetArgIlePro     140145150     SerThrLeuValAspLeuLeuAlaGlyGlyHisTrpGlyValLeu     155160165     ValGlyLeuAlaTyrPheSerMetGlnAlaAsnTrpAlaLysVal     170175180     IleLeuValLeuPheLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:94:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: Z7     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:94:     ValAsnTyrHisAsnAlaSerGlyValTyrHisIleThrAsnAsp     51015     CysProAsnSerSerIleMetTyrGluAlaGluHisHisIleLeu     202530     HisLeuProGlyCysValProCysValArgGluGlyAsnGlnSer     354045     ArgCysTrpValAlaLeuThrProThrValAlaAlaProTyrIle     505560     GlyAlaProLeuGluSerIleArgArgHisValAspLeuMetVal     657075     GlyAlaAlaThrValCysSerAlaLeuTyrIleGlyAspLeuCys     808590     GlyGlyValPheLeuValGlyGlnMetPheSerPheGlnProArg     95100105     ArgHisTrpThrThrGlnAspCysAsnCysSerIleTyrAlaGly     110115120     HisValThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrThrLeuValLeuAlaGlnValMetArgIlePro     140145150     SerThrLeuValAspLeuLeuThrGlyGlyHisTrpGlyIleLeu     155160165     IleGlyValAlaTyrPheCysMetGlnAlaAsnTrpAlaLysVal     170175180     IleLeuValLeuPheLeuTyrAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:95:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: DK13     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:95:     TyrAsnTyrArgAsnSerSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluThrAspTyrHisIleLeu     202530     HisLeuProGlyCysValProCysValArgGluGlyAsnLysSer     354045     ThrCysTrpValSerLeuThrProThrValAlaAlaGlnHisLeu     505560     AsnAlaProLeuGluSerLeuArgArgHisValAspLeuMetVal     657075     GlyGlyAlaThrLeuCysSerAlaLeuTyrIleGlyAspValCys     808590     GlyGlyValPheLeuValGlyGlnLeuPheThrPheGlnProArg     95100105     ArgHisTrpThrThrGlnAspCysAsnCysSerIleTyrThrGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrAlaThrLeuValLeuAlaGlnLeuMetArgIlePro     140145150     GlyAlaMetValAspLeuLeuAlaGlyGlyHisTrpGlyIleLeu     155160165     ValGlyIleAlaTyrPheSerMetGlnAlaAsnTrpAlaLysVal     170175180     IleLeuValLeuPheLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:96:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA1     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:96:     ValProTyrArgAsnAlaSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAspSerLeuIleLeu     202530     HisAlaProGlyCysValProCysValArgGlnAspAsnValSer     354045     ArgCysTrpValGlnIleThrProThrLeuSerAlaProThrPhe     505560     GlyAlaValThrAlaProLeuArgArgAlaValAspTyrLeuAla     657075     GlyGlyAlaAlaLeuCysSerAlaLeuTyrValGlyAspAlaCys     808590     GlyAlaValPheLeuValGlyGlnMetPheThrTyrArgProArg     95100105     GlnHisThrThrValGlnAspCysAsnCysSerIleTyrSerGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuLeuMetAlaGlnMetLeuArgIlePro     140145150     GlnValValIleAspIleIleAlaGlyGlyHisTrpGlyValLeu     155160165     PheAlaAlaAlaTyrPheAlaSerAlaAlaAsnTrpAlaLysVal     170175180     ValLeuValLeuPheLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:97:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA4     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:97:     ValProTyrArgAsnAlaSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAspAsnLeuIleLeu     202530     HisAlaProGlyCysValProCysValArgGlnAspAsnValSer     354045     LysCysTrpValGlnIleThrProThrLeuSerAlaProAsnLeu     505560     GlyAlaValThrAlaProLeuArgArgAlaValAspTyrLeuAla     657075     GlyGlyAlaAlaLeuCysSerAlaLeuTyrValGlyAspAlaCys     808590     GlyAlaValPheLeuValGlyGlnMetPheThrTyrArgProArg     95100105     GlnHisThrThrValGlnAspCysAsnCysSerIleTyrSerGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuLeuMetAlaGlnLeuLeuArgIlePro     140145150     GlnValValIleAspIleIleAlaGlyGlyHisTrpGlyValLeu     155160165     PheAlaAlaAlaTyrPheAlaSerAlaAlaAsnTrpAlaLysVal     170175180     IleLeuValLeuPheLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:98:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA5     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:98:     ValProTyrArgAsnAlaSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAspAsnLeuIleLeu     202530     HisAlaProGlyCysValProCysValLysGluGlyAsnValSer     354045     ArgCysTrpValGlnIleThrProThrLeuSerAlaProAsnLeu     505560     GlyAlaValThrAlaProLeuArgArgValValAspTyrLeuAla     657075     GlyGlyAlaAlaLeuCysSerAlaLeuTyrValGlyAspAlaCys     808590     GlyAlaValPheLeuValGlyGlnMetPheThrTyrArgProArg     95100105     GlnHisThrThrValGlnAspCysAsnCysSerIleTyrSerGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValMetAlaGlnValLeuArgIlePro     140145150     GlnValValIleAspIleIleAlaGlyGlyHisTrpGlyValLeu     155160165     PheAlaValAlaTyrPheAlaSerAlaAlaAsnTrpAlaLysVal     170175180     ValLeuValLeuPheLeuPheAlaGlyValAspGly     185190     (2) INFORMATION FOR SEQ ID NO:99:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA6     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:99:     ValProTyrArgAsnAlaSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAspAspLeuIleLeu     202530     HisAlaProGlyCysValProCysValArgLysAspAsnValSer     354045     ArgCysTrpValHisIleThrProThrLeuSerAlaProSerLeu     505560     GlyAlaValThrAlaProLeuArgArgAlaValAspTyrLeuAla     657075     GlyGlyAlaAlaLeuCysSerAlaLeuTyrValGlyAspValCys     808590     GlyAlaLeuPheLeuValGlyGlnMetPheThrTyrArgProArg     95100105     GlnHisAlaThrValGlnAspCysAsnCysSerIleTyrSerGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProAlaThrAlaLeuValMetAlaGlnMetLeuArgIlePro     140145150     GlnValValIleAspIleIleAlaGlyGlyHisTrpGlyValLeu     155160165     PheAlaAlaAlaTyrPheAlaSerAlaAlaAsnTrpAlaLysVal     170175180     ValLeuValLeuPheLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:100:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA7     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:100:     ValProTyrArgAsnAlaSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAspAsnLeuIleLeu     202530     HisAlaProGlyCysValProCysValArgGlnAsnAsnValSer     354045     ArgCysTrpValGlnIleThrProThrLeuSerAlaProAsnLeu     505560     GlyAlaValThrAlaProLeuArgArgAlaValAspTyrLeuAla     657075     GlyGlyAlaAlaLeuCysSerAlaLeuTyrValGlyAspAlaCys     808590     GlyAlaValPheLeuValGlyGlnMetPheSerTyrArgProArg     95100105     GlnHisThrThrValGlnAspCysAsnCysSerIleTyrSerGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValMetAlaGlnLeuLeuArgIlePro     140145150     GlnValValIleAspIleIleAlaGlyGlyHisTrpGlyValLeu     155160165     PheAlaAlaAlaTyrPheAlaSerAlaAlaAsnTrpAlaLysVal     170175180     ValLeuValLeuPheLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:101:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: SA13     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:101:     ValProTyrArgAsnAlaSerGlyValTyrHisValThrAsnAsp     51015     CysProAsnSerSerIleValTyrGluAlaAspAspLeuIleLeu     202530     HisAlaProGlyCysValProCysValArgGlnGlyAsnValSer     354045     ArgCysTrpValGlnIleThrProThrLeuSerAlaProSerLeu     505560     GlyAlaValThrAlaProLeuArgArgAlaValAspTyrLeuAla     657075     GlyGlyAlaAlaLeuCysSerAlaLeuTyrValGlyAspAlaCys     808590     GlyAlaValPheLeuValGlyGlnMetPheThrTyrSerProArg     95100105     ArgHisAsnValValGlnAspCysAsnCysSerIleTyrSerGly     110115120     HisIleThrGlyHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrAlaLeuValMetAlaGlnLeuLeuArgIlePro     140145150     GlnValValIleAspIleIleAlaGlyAlaHisTrpGlyValLeu     155160165     PheAlaAlaAlaTyrTyrAlaSerAlaAlaAsnTrpAlaLysVal     170175180     ValLeuValLeuPheLeuPheAlaGlyValAspAla     185190     (2) INFORMATION FOR SEQ ID NO:102:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 192 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (vi) ORIGINAL SOURCE:     (A) ORGANISM: homosapiens     (C) INDIVIDUAL ISOLATE: HK2     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:102:     LeuThrTyrGlnAsnSerSerGlnLeuTyrHisLeuThrAsnAsp     11015     CysProAsnSerSerIleValLeuGluAlaAspAlaMetIleLeu     202530     HisLeuProGlnCysLeuProCysValArgValAspAspArgSer     354045     ThrCysTrpHisAlaValThrProThrLeuAlaIleProAsnAla     505560     SerThrProAlaThrGlnPheArgArgHisValAspLeuLeuAla     657075     GlnAlaAlaValValCysSerSerLeuTyrIleGlnAspLeuCys     808590     GlnSerLeuPheLeuAlaGlnGlnLeuPheThrPheGlnProArg     95100105     ArgHisTrpThrValGlnAspCysAsnCysSerIleTyrThrGln     110115120     HisValThrGlnHisArgMetAlaTrpAspMetMetMetAsnTrp     125130135     SerProThrThrThrLeuValLeuSerSerIleLeuArgValPro     140145150     GluIleCysAlaSerValIlePheGlnGlnHisTrpGlnIleLeu     155160165     LeuAlaValAlaTyrPheGlnMetAlaGlnAsnTrpLeuLysVal     170175180     LeuAlaValLeuPheLeuPheAlaGlnValGluAla     185190     (2) INFORMATION FOR SEQ ID NO:103:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:103:     GCGTCCGGGTTCTGGAAGACGGCGTGAACTATGCAACAGG40     (2) INFORMATION FOR SEQ ID NO:104:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:104:     AGGCTTTCATTGCAGTTCAAGGCCGTGCTATTGATGTGCC40     (2) INFORMATION FOR SEQ ID NO:105:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:105:     AAGACGGCGTGAACTATGCAACAGGGAACCTTCCTGGTTG40     (2) INFORMATION FOR SEQ ID NO:106:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:106:     AGTTCAAGGCCGTGCTATTGATGTGCCAACTGCCGTTGGT40     (2) INFORMATION FOR SEQ ID NO:107:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:107:     AAGACGGCGTGAATTCTGCAACAGGGAACCTTCCTGGTTG40     (2) INFORMATION FOR SEQ ID NO:108:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:108:     AGTTCAAGGCCGTGGAATTCATGTGCCAACTGCCGTTGGT40     (2) INFORMATION FOR SEQ ID NO:109:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 42 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:109:     ARCTYCGACGTYACATCGAYCTGCTYGTYGGRAGYGCCACCC42     (2) INFORMATION FOR SEQ ID NO:110:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 31 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:110:     RCARGCCRTCTTGGAYATGATCGCTGGWGCY31     (2) INFORMATION FOR SEQ ID NO:111:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 42 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:111:     CRATACGACRYCAYGTCGAYTTGCTCGTTGGGGCGGCTRYYT42     (2) INFORMATION FOR SEQ ID NO:112:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 31 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:112:     RCAAGCTRTCRTGGAYRTGGTRRCRGGRGCC31     (2) INFORMATION FOR SEQ ID NO:113:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:113:     TTGCGGACKCACATYGACATGGTYGTGATGTCCGCCACGC40     (2) INFORMATION FOR SEQ ID NO:114:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 43 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:114:     GATGCGCGTTCCCGAGGTCATCWTAGACATCRTYRGCGGRGCD43     (2) INFORMATION FOR SEQ ID NO:115:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 54 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:115:     AATGGCACCYTGCRCTGCTGGATACAAGTRACACCTAATGTGGCTGTGAA50     ACAC54     (2) INFORMATION FOR SEQ ID NO:116:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 31 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:116:     TGARCTAGYCCTYSARGTYGTCTTCGGYGGY31     (2) INFORMATION FOR SEQ ID NO:117:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 54 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:117:     GCCAACGTCTCTCGATGTTGGGTGCCGGTTGCCCCCAATCTCGCCATAAG50     TCAA54     (2) INFORMATION FOR SEQ ID NO:118:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 46 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:118:     AAGGGCCTGCGAGCACACATCGATATCATCGTGATGTCTGCTACGG46     (2) INFORMATION FOR SEQ ID NO:119:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 45 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:119:     TTGGTGCGCATCCCGGAAGTCATCTTGGATATTGTTACAGGAGGT45     (2) INFORMATION FOR SEQ ID NO:120:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:120:     AGTCAGGTAYGTCGGAGCAACCACCGCYTCGATACGCAGT40     (2) INFORMATION FOR SEQ ID NO:121:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 46 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:121:     AGCCTTCACGTTCAGACCKCGTCGCCATCAAACRGTCCAGACCTGT46     (2) INFORMATION FOR SEQ ID NO:122:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 75 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:122:     TCCCCCGCYGTGGGTATGGTGGTRGCGCACRTYCTGCGDYTGCCCCAGAC50     CKTGTTYGACATAMTRGCYGGGGCC75     (2) INFORMATION FOR SEQ ID NO:123:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 39 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:123:     ACGCCGGTGACGCCTACAGTGGCTGTCGCACACCCGGGC39     (2) INFORMATION FOR SEQ ID NO:124:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 42 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:124:     ATGAGGGTCCCCACAGCCTTTCTCGACATGGTTGCCGGAGGC42     (2) INFORMATION FOR SEQ ID NO:125:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:125:     CGCGCCCTATCCCAACGCACCGTTAGAGTCCATGCGCAGG40     (2) INFORMATION FOR SEQ ID NO:126:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 49 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:126:     TCAGATCTTACGGATCCCCTCTATCCTAGGTGACTTGCTCACCGGGGGT49     (2) INFORMATION FOR SEQ ID NO:127:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 54 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:127:     CAGTCACGCTGCTGGGTGGCCCTTACTCCCACCGTGGCGGYGYCTTATAT50     CGGT54     (2) INFORMATION FOR SEQ ID NO:128:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 31 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:128:     TAGCACTCTGGTRGAYCTACTCRCTGGAGGG31     (2) INFORMATION FOR SEQ ID NO:129:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 54 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:129:     AAGTCTACATGCTGGGTGTCTCTCACCCCCACCGTGGCTGCGCAACATCT50     GAAT54     (2) INFORMATION FOR SEQ ID NO:130:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 31 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:130:     AGGCGCCATGGTCGACCTGCTTGCAGGCGGC31     (2) INFORMATION FOR SEQ ID NO:131:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 43 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:131:     TCAGCCCCGAVYYTCGGAGCGGTCACGGCTCCTCTTCGGAGGG43     (2) INFORMATION FOR SEQ ID NO:132:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 44 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:132:     TGYTACGGATYCCCCARGTGGTCATHGACATCATWGCCGGGGSC44     (2) INFORMATION FOR SEQ ID NO:133:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 40 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:133:     CATACCAAATGCTTCCACGCCCGCAACGGGATTCCGCAGG40     (2) INFORMATION FOR SEQ ID NO:134:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 37 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:134:     TCTTCTTGCGGGCGCCGCAGTGGTTTGCTCATCCCTG37     (2) INFORMATION FOR SEQ ID NO:135:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 52 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:135:     ATCTAGCATCTTGAGGGTACCTGAGATTTGTGCGAGTGTGATATTTGGTG50     GC52     (2) INFORMATION FOR SEQ ID NO:136:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:136:     TrpIleGlnValThrProAsnValAlaValLysHisArgGlyAla     51015     LeuThrHisAsnLeuArgXaaHisXaaAspXaaIleValMetAla     202530     AlaThrVal     (2) INFORMATION FOR SEQ ID NO:137:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:137:     TrpValProValAlaProAsnLeuAlaIleSerGlnProGlyAla     51015     LeuThrLysGlyLeuArgAlaHisIleAspIleIleValMetSer     202530     AlaThrVal     (2) INFORMATION FOR SEQ ID NO:138:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:138:     TrpIleProValXaaProAsnValAlaValXaaXaaProGlyAla     51015     LeuThrGlnGlyLeuArgThrHisIleAspMetValValMetSer     202530     AlaThrLeu     (2) INFORMATION FOR SEQ ID NO:139:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:139:     TrpThrXaaValThrProThrValAlaValArgTyrValGlyAla     51015     ThrThrAlaSerIleArgSerHisValAspLeuLeuValGlyAla     202530     AlaThrXaa     (2) INFORMATION FOR SEQ ID NO:140:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:140:     TrpValAlaLeuXaaProThrLeuAlaAlaArgAsnXaaXaaXaa     51015     XaaThrXaaXaaIleArgXaaHisValAspLeuLeuValGlyAla     202530     AlaXaaPhe     (2) INFORMATION FOR SEQ ID NO:141:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:141:     TrpValXaaXaaXaaProThrValAlaThrArgAspGlyLysLeu     51015     ProXaaXaaGlnLeuArgArgXaaIleAspLeuLeuValGlySer     202530     AlaThrLeu     (2) INFORMATION FOR SEQ ID NO:142:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:142:     TrpThrProValThrProThrValAlaValAlaHisProGlyAla     51015     ProLeuGluSerPheArgArgHisValAspLeuMetValGlyAla     202530     AlaThrLeu     (2) INFORMATION FOR SEQ ID NO:143:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:143:     TrpValAlaLeuThrProThrValAlaXaaXaaTyrIleGlyAla     51015     ProLeuXaaSerXaaArgArgHisValAspLeuMetValGlyAla     202530     AlaThrVal     (2) INFORMATION FOR SEQ ID NO:144:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:144:     TrpValSerLeuThrProThrValAlaAlaGlnHisLeuAsnAla     51015     ProLeuGluSerLeuArgArgHisValAspLeuMetValGlyGly     202530     AlaThrLeu     (2) INFORMATION FOR SEQ ID NO:145:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:145:     TrpValProLeuThrProThrValAlaAlaProTyrProAsnAla     51015     ProLeuGluSerMetArgArgHisValAspLeuMetValGlyAla     202530     AlaThrMet     (2) INFORMATION FOR SEQ ID NO:146:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:146:     TrpValXaaIleThrProThrLeuSerAlaProXaaXaaGlyAla     51015     ValThrAlaProLeuArgArgXaaValAspTyrLeuAlaGlyGly     202530     AlaAlaLeu     (2) INFORMATION FOR SEQ ID NO:147:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 33 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:147:     TrpHisAlaValThrProThrLeuAlaIleProAsnAlaSerThr     51015     ProAlaThrGlyPheArgArgHisValAspLeuLeuAlaGlyAla     202530     AlaValVal     (2) INFORMATION FOR SEQ ID NO:148:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:148:     ThrLeuThrMetIleLeuAlaTyrAlaAlaArgValProGluLeu     51015     XaaLeuXaaValValPheGlyGly     20     (2) INFORMATION FOR SEQ ID NO:149:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:149:     ThrThrThrMetLeuLeuAlaTyrLeuValArgIleProGluVal     51015     IleLeuAspIleValThrGlyGly     20     (2) INFORMATION FOR SEQ ID NO:150:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:150:     ThrXaaThrXaaIleLeuAlaTyrXaaMetArgValProGluVal     51015     IleXaaAspIleXaaXaaGlyAla     20     (2) INFORMATION FOR SEQ ID NO:151:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:151:     AlaValGlyMetValValAlaHisXaaLeuArgLeuProGlnThr     51015     XaaPheAspIleXaaAlaGlyAla     20     (2) INFORMATION FOR SEQ ID NO:152:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:152:     ThrXaaAlaLeuValXaaSerGlnLeuLeuArgXaaProGlnAla     51015     XaaXaaAspXaaValXaaGlyAla     20     (2) INFORMATION FOR SEQ ID NO:153:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:153:     ThrXaaAlaLeuValXaaAlaGlnLeuLeuArgXaaProGlnAla     51015     XaaLeuAspMetIleAlaGlyAla     20     (2) INFORMATION FOR SEQ ID NO:154:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:154:     ThrThrThrLeuLeuLeuAlaGlnIleMetArgValProThrAla     51015     PheLeuAspMetValAlaGlyGly     20     (2) INFORMATION FOR SEQ ID NO:155:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:155:     ThrThrThrLeuXaaLeuAlaGlnValMetArgIleProSerThr     51015     LeuValAspLeuLeuXaaGlyGly     20     (2) INFORMATION FOR SEQ ID NO:156:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:156:     ThrAlaThrLeuValLeuAlaGlnLeuMetArgIleProGlyAla     51015     MetValAspLeuLeuAlaGlyGly     20     (2) INFORMATION FOR SEQ ID NO:157:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:157:     ThrSerAlaLeuIleMetAlaGlnIleLeuArgIleProSerIle     51015     LeuGlyAspLeuLeuThrGlyGly     20     (2) INFORMATION FOR SEQ ID NO:158:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:158:     XaaThrAlaLeuXaaMetAlaGlnXaaLeuArgIleProGlnVal     51015     ValIleAspIleIleAlaGlyXaa     20     (2) INFORMATION FOR SEQ ID NO:159:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 23 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS: unknown     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:159:     ThrThrThrLeuValLeuSerSerIleLeuArgValProGluIle     51015     CysAlaSerValIlePheGlyGly     20 

We claim:
 1. A method for detecting the presence of hepatitis C virus comprising:(a) amplifying RNA via reverse transcription-polymerase chain reaction to produce amplification products; (b) contacting said products with a nucleotide sequence selected from the sequences shown in SEQ ID NO: 1-51; and (c) detecting complexes of said products and said nucleotide sequence.
 2. A method for detecting the presence of hepatitis C virus comprising:(a) amplifying RNA via a reverse transcription-polymerase chain reaction process using primers selected from the group consisting of SEQ ID NO.: 103 through SEQ ID NO. 108; and (b) detecting said amplification product.
 3. Isolated primers, wherein said primers have nucleic acid sequences selected from the group consisting of SEQ ID NO: 103 through SEQ ID NO:
 108. 4. A diagnostic kit for use in detecting the presence of hepatitus C virus, said kit comprising: primers having nucleic acid sequences selected from the group consisting of SEQ ID NO:103 through SEQ ID NO:108.
 5. A method for determining the genotype of a hepatitis C virus, said method comprising:(a) amplifying RNA via reverse transcription-polymerase chain reaction to produce amplification products; (b) contacting said products with at least one E1 region genotype-specific oligonucleotide; and (c) detecting complexes of said products and said oligonucleotide(s).
 6. The method of claim 5, wherein said amplification of step (a) uses primers having sequences according to SEQ ID NO:103 through SEQ ID NO:108.
 7. The method of claim 6, wherein said oligonucleotide of step (b) is a nucleic acid sequence selected from the group consisting of SEQ ID NO:109 through SEQ ID NO:135.
 8. Isolated genotype-specific oligonucleotides having nucleic acid sequences deduced from genotype-specific nucleic acid domains located in SEQ ID Nos: 1 through 51 or in consensus sequences produced from at least two sequences shown in SEQ ID Nos: 1 through
 51. 9. Isolated genotype-specific oligonucleotides having nucleic acid sequences selected from the group consisting of SEQ ID NO: 109 through SEQ ID NO:
 135. 10. A diagnostic kit for determining the genotype of a hepatitis C virus, said kit comprising primers selected from the group consisting of SEQ ID NO:103 through SEQ ID NO:108 and hybridization probes selected from the group consisting of SEQ ID NO:109 through SEQ ID NO:135. 